ATAC测序：一种全基因组范围内检测染色质可及性的方法。
ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide.
作者信息
Buenrostro Jason D, Wu Beijing, Chang Howard Y, Greenleaf William J
机构信息
Department of Genetics, Stanford University School of Medicine, Stanford, California.
Program in Epithelial Biology and the Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California.
出版信息
Curr Protoc Mol Biol. 2015 Jan 5;109:21.29.1-21.29.9. doi: 10.1002/0471142727.mb2129s109.
This unit describes Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin. Sequencing reads can then be used to infer regions of increased accessibility, as well as to map regions of transcription-factor binding and nucleosome position. The method is a fast and sensitive alternative to DNase-seq for assaying chromatin accessibility genome-wide, or to MNase-seq for assaying nucleosome positions in accessible regions of the genome.
本单元介绍了用于全基因组染色质可及性图谱绘制的高通量测序转座酶可及染色质分析方法（ATAC-seq）。该方法利用超活性Tn5转座酶探测DNA可及性，将测序接头插入染色质的可及区域。测序读数可用于推断可及性增加的区域，以及绘制转录因子结合区域和核小体位置图谱。该方法是一种快速且灵敏的替代方法，可替代DNase-seq用于全基因组染色质可及性分析，或替代MNase-seq用于分析基因组可及区域中的核小体位置。
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