Yamamoto A, Sakane T, Kawaratani T, Aisaka A, Hashida M, Sezaki H
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Life Sci. 1989;44(2):121-30. doi: 10.1016/0024-3205(89)90529-8.
Intestinal uptake of p-aminobenzoic acid was examined by means of an in vitro everted sac technique in rats immunized with ovalbumin-p-aminobenzoic acid conjugate. A dose-dependent and antigen-specific decrease in the serosal transfer of p-aminobenzoic acid was observed in rats immunized 6 times with protein-hapten conjugate compared with the control. There was a significant increase in the recovery of p-acetamidobenzoic acid, a metabolite of p-aminobenzoic acid, in mucosal fluid, tissue, and serosal fluid in the jejunum. In the case of ileum, increase of p-acetamidobenzoic acid was observed in mucosal fluid. However, there was no significant effect in the ileal p-acetamidobenzoic acid in tissue and serosal fluid between immunized and non-immunized rats. To examine the increased metabolism of immunized rats, N-acetyltransferase activity of the small intestinal mucosa was examined. There was a significant increase in mucosal N-acetyltransferase activity in immunized rats compared with the control animals. These observations suggested that the mucosal immune system may play an important role in regulating the intestinal uptake of the low molecular weight compounds.
采用体外翻转囊技术，对用卵清蛋白 - 对氨基苯甲酸结合物免疫的大鼠肠道摄取对氨基苯甲酸的情况进行了研究。与对照组相比，用蛋白 - 半抗原结合物免疫6次的大鼠，对氨基苯甲酸的浆膜转运呈剂量依赖性且抗原特异性降低。空肠黏膜液、组织和浆膜液中对氨基苯甲酸的代谢产物对乙酰氨基苯甲酸的回收率显著增加。在回肠中，黏膜液中观察到对乙酰氨基苯甲酸增加。然而，免疫大鼠和未免疫大鼠之间，回肠组织和浆膜液中的对乙酰氨基苯甲酸没有显著差异。为了研究免疫大鼠代谢增加的情况，检测了小肠黏膜的N - 乙酰转移酶活性。与对照动物相比，免疫大鼠的黏膜N - 乙酰转移酶活性显著增加。这些观察结果表明，黏膜免疫系统可能在调节低分子量化合物的肠道摄取中起重要作用。
