Yamamoto A, Kawaratani T, Kawashima K, Hashida M, Sezaki H
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Pharm Res. 1990 Jul;7(7):767-71. doi: 10.1023/a:1015832009217.
Intestinal transport of sulfanilic acid was examined by means of an in vitro everted sac technique in rats immunized with a bovine gamma-globulin-sulfanilic acid conjugate. At a low concentration of sulfanilic acid, the intestinal transport of sulfanilic acid was decreased in rats immunized with bovine gamma-globulin-sulfanilic acid conjugate. This phenomenon was dose dependent and antigen specific, since there was no difference in the transport of sulfanilic acid at a high concentration and of an unrelated hapten. These results suggested that parenteral immunization impaired not only the intestinal transport of macromolecular antigens, as previously shown, but also the transport of the low molecular weight hapten, sulfanilic acid.
采用体外翻囊技术，在经牛γ球蛋白 - 对氨基苯磺酸偶联物免疫的大鼠中检测对氨基苯磺酸的肠道转运。在低浓度对氨基苯磺酸时，经牛γ球蛋白 - 对氨基苯磺酸偶联物免疫的大鼠中对氨基苯磺酸的肠道转运减少。这种现象具有剂量依赖性和抗原特异性，因为在高浓度对氨基苯磺酸和无关半抗原的转运方面没有差异。这些结果表明，正如先前所示，胃肠外免疫不仅损害大分子抗原的肠道转运，还损害低分子量半抗原对氨基苯磺酸的转运。
