Erster Oran, Roth Asael, King Roni, Markovics Alex
Division of Parasitology, Kimron Veterinary Institute, P.O. Box 12, Bet Dagan 50250, Israel.
Division of Parasitology, Kimron Veterinary Institute, P.O. Box 12, Bet Dagan 50250, Israel.
Vet Parasitol. 2016 Nov 15;231:128-131. doi: 10.1016/j.vetpar.2016.10.023. Epub 2016 Nov 4.
Trichinellosis is a worldwide disease caused by nematode worms of the genus Trichinella, frequently diagnosed in Israel. However, the identity of the Israeli isolates have not been studied. Here we describe the molecular characterization of 58 isolates collected from jackals (Canis aureus), wild boar (Sus scrofa), foxes (Vulpes vulpes) and a wolf (Canis lupus) in central and northern Israel. Isolates were analyzed using the multiplex PCR analysis encompassing expansion segment V (ESV) and internal sequence 1 (ITS-1) markers, which identified 52 of the 58 samples. Out of the six unidentified samples, four were successfully identified using extended PCR assays for ESV and ITS-1, developed in this study. Our analysis identified 44 isolates as T. britovi, 8 as T. spiralis, four mixed infections, and two isolates were not identified. Clonal analysis of the ITS-1 sequences from six isolates confirmed the initial identification of four mixed infections. These results show that the prevalent species in Israel are T. britovi and T. spiralis, with nearly 7% (4 of 58) incidence of mixed infection.
旋毛虫病是一种由旋毛虫属线虫引起的全球性疾病，在以色列经常被诊断出来。然而，以色列分离株的身份尚未得到研究。在此，我们描述了从以色列中部和北部的豺（金豺）、野猪（野猪）、狐狸（赤狐）和一只狼（灰狼）身上收集的58株分离株的分子特征。使用包含扩增片段V（ESV）和内部序列1（ITS-1）标记的多重PCR分析对分离株进行分析，该分析鉴定出58个样本中的52个。在6个未鉴定的样本中，有4个通过本研究开发的针对ESV和ITS-1的扩展PCR检测成功鉴定。我们的分析确定44株分离株为布氏旋毛虫，8株为旋毛形线虫，4株为混合感染，2株分离株未鉴定。对6株分离株的ITS-1序列进行克隆分析，证实了最初对4例混合感染的鉴定。这些结果表明，以色列流行的物种是布氏旋毛虫和旋毛形线虫，混合感染发生率近7%（58例中的4例）。
