Lago María, Bandín Isabel, Olveira José G, Dopazo Carlos P
Departamento de Microbiología y Parasitología, Instituto de Acuicultura-Universidade de Santiago de Compostela, Santiago de Compostela 15706, Spain.
Virology. 2017 Jan 15;501:1-11. doi: 10.1016/j.virol.2016.11.003. Epub 2016 Nov 10.
Reassortment is one of the main mechanisms of evolution in dsRNA viruses with segmented genomes. It contributes to generate genetic diversity and plays an important role in the emergence and spread of new strains with altered virulence. Natural reassorment has been demonstrated among infectious pancreatic necrosis-like viruses (genus Aquabirnavirus, Birnaviridae). In the present study, coinfections between different viral strains, and genome sequencing by the Sanger and Illumina methods were applied to analyze the frequency of reassortment of this virus in vitro, the possible mechanisms involved, and its effect on virulence. Results have demonstrated that reassortment is a cell-dependent and non-random process, probably through differential expression of the different mRNA classes in the ribosomes of a specific cell, and by specific associations between the components to construct the ribonucleoprotein (RNP) complexes and/or RNP cross-inhibition. However, the precise mechanisms involved, known in other viruses, still remain to be demonstrated in birnaviruses.
重配是具有分段基因组的双链RNA病毒进化的主要机制之一。它有助于产生遗传多样性，并在毒力改变的新毒株的出现和传播中发挥重要作用。在传染性胰腺坏死样病毒（水 birnavirus 属，Birnaviridae）之间已证实存在自然重配。在本研究中，应用不同病毒株之间的共感染以及通过桑格法和Illumina法进行基因组测序，以分析该病毒在体外的重配频率、涉及的可能机制及其对毒力的影响。结果表明，重配是一个细胞依赖性且非随机的过程，可能是通过特定细胞核糖体中不同mRNA类别的差异表达，以及通过构建核糖核蛋白（RNP）复合物的组分之间的特定关联和/或RNP交叉抑制。然而，在其他病毒中已知的具体机制在 birnavirus 中仍有待证实。
