Zheng Guodong, Chao Yingxin, Luo Meixia, Xie Bin, Zhang Dedong, Hu Pingjun, Yang Xiujuan, Yang Depo, Wei Minyan
Key Laboratory of Molecular Target & Clinical Pharmacology and the State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
The Affiliated Brain Hospital of Guangzhou Medical University, Guangzhou Huiai Hospital, Guangzhou, China.
Evid Based Complement Alternat Med. 2020 Mar 3;2020:4736152. doi: 10.1155/2020/4736152. eCollection 2020.
Citri Reticulatae Pericarpium (CRP), known as Chenpi (CP) in Chinese, is a medicinal food for health and fitness. In order to find out the characteristic activity chemicals distinguishing various cultivars of CRP and provide a reference for effective development of citrus resources, an "activity fingerprint" of CRP from 21 different cultivars was established based on the evaluation of antitussive and expectorant activities. There were 18 common peaks in the HPLC fingerprint, of which 3 flavonoid glycosides and 14 polymethoxyflavonoids (PMFs) were identified by LC/MS-IT-TOF. Furthermore, five characteristic chemicals were determined and similarity calculation with principal component analysis (PCA) was combined together to compare the similarity and difference among various cultivars. The results showed that some other cultivars were also similar to . "Chachi" except for . "Tangerina" and . "Dahongpao" recorded in Chinese Pharmacopoeia. Most importantly, the peels of . "Shiyueju," . "Ponkan," . "Tribute," and . "Bayueju," traditionally rarely used for medicinal food, were highly similar to that of . "Chachi" and rich in bioactive flavonoids, which can be considered the effective medicinal resources of CRP.
陈皮是一种药食同源的保健食品。为了找出区分不同陈皮品种的特征活性成分，并为柑橘资源的有效开发提供参考，基于镇咳祛痰活性评价建立了21个不同品种陈皮的“活性指纹图谱”。高效液相色谱指纹图谱中有18个共有峰，通过液相色谱-质谱-离子阱-飞行时间质谱鉴定出其中3种黄酮苷和14种多甲氧基黄酮（PMFs）。此外，确定了5种特征性成分，并结合主成分分析（PCA）进行相似度计算，以比较不同品种之间的异同。结果表明，除《中国药典》记载的“茶枝柑”“橘”“大红袍”外，其他一些品种也与“茶枝柑”相似。最重要的是，传统上很少用作药食的“十月橘”“椪柑”“贡柑”和“八月橘”的果皮与“茶枝柑”高度相似，且富含生物活性黄酮类化合物，可被视为陈皮的有效药用资源。
