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1
Cloning of phenotypically different human lymphocytes originating from a single stem cell.源自单个干细胞的表型不同的人类淋巴细胞的克隆。
J Exp Med. 1989 Apr 1;169(4):1265-76. doi: 10.1084/jem.169.4.1265.
2
Molecular analyses of in vivo hprt mutations in human T-lymphocytes: IV. Studies in newborns.人类T淋巴细胞体内次黄嘌呤磷酸核糖转移酶（hprt）突变的分子分析：IV. 新生儿研究
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3
Molecular analyses of in vivo hprt mutant T cells from atomic bomb survivors.原子弹爆炸幸存者体内hprt突变T细胞的分子分析。
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Transplantable progenitors of natural killer cells are distinct from those of T and B lymphocytes.自然杀伤细胞的可移植祖细胞与T淋巴细胞和B淋巴细胞的可移植祖细胞不同。
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Mutant frequency at the HPRT locus in peripheral blood T-lymphocytes of atomic bomb survivors.原子弹爆炸幸存者外周血T淋巴细胞中次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HPRT）基因座的突变频率。
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T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood.通过T细胞克隆检测人类外周血中存在的对6-硫鸟嘌呤耐药的突变淋巴细胞。
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Stem cells, clonal progenitors, and commitment to the three lymphocyte lineages: T, B, and NK cells.
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High-dose radiation preferentially induces the clonal expansion of hematopoietic progenitor cells over mature T and B cells in mouse bone marrow.高剂量辐射优先诱导小鼠骨髓中造血祖细胞而非成熟T细胞和B细胞的克隆性扩增。
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Relationship between spontaneous γH2AX foci formation and progenitor functions in circulating hematopoietic stem and progenitor cells among atomic-bomb survivors.原子弹爆炸幸存者循环造血干细胞和祖细胞中自发γH2AX焦点形成与祖细胞功能之间的关系。
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Selection against blood cells deficient in hypoxanthine phosphoribosyltransferase (HPRT) in Lesch-Nyhan heterozygotes occurs at the level of multipotent stem cells.在莱施-奈恩杂合子中，针对缺乏次黄嘌呤磷酸核糖基转移酶（HPRT）的血细胞的选择发生在多能干细胞水平。
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7
Inhibition of human immunodeficiency virus replication in a human T cell line by antisense RNA expressed in the cell.细胞内表达的反义RNA对人T细胞系中人类免疫缺陷病毒复制的抑制作用
Virus Genes. 1991 Jul;5(3):189-202. doi: 10.1007/BF00568969.

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Evidence that essential thrombocythemia is a clonal disorder with origin in a multipotent stem cell.原发性血小板增多症是一种起源于多能干细胞的克隆性疾病的证据。
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T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood.通过T细胞克隆检测人类外周血中存在的对6-硫鸟嘌呤耐药的突变淋巴细胞。
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A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains.一个人类T细胞特异性cDNA克隆编码一种与免疫球蛋白链具有广泛同源性的蛋白质。
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Relationship between human T cell functional heterogeneity and human T cell surface molecules.人类T细胞功能异质性与人类T细胞表面分子之间的关系。
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Organization of the HPRT gene and related sequences in the human genome.人类基因组中次黄嘌呤磷酸核糖基转移酶（HPRT）基因及相关序列的组织
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源自单个干细胞的表型不同的人类淋巴细胞的克隆。

Cloning of phenotypically different human lymphocytes originating from a single stem cell.

作者信息

Hakoda M, Hirai Y, Shimba H, Kusunoki Y, Kyoizumi S, Kodama Y, Akiyama M

机构信息

Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.

出版信息

J Exp Med. 1989 Apr 1;169(4):1265-76. doi: 10.1084/jem.169.4.1265.

DOI:10.1084/jem.169.4.1265
PMID:2784484
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2189249/
Abstract

By using hypoxanthine guanine phosphoribosyltransferase (hprt) gene alterations and chromosome aberrations as in vivo cellular markers, human T, NK, and B cells originating from a single stem cell have been successfully cloned from the peripheral blood of an atomic bomb survivor from Hiroshima. These mutant lymphocytes were selectively cloned, taking advantage of their resistance to a purine analogue, 6-thioguanine. The cloned lymphocytes possessed the same hprt gene alterations and the same chromosome aberration (20q-), but exhibited different surface or functional phenotypes and different rearrangements of TCR or Ig genes. The chromosome aberration patterns strongly suggested that the original stem cell initiated differentiation into each cell type after exposure to atomic bomb radiation. Since the person studied here was exposed to the bomb at 17 yr age, the results suggested that common stem cells exist in adults for at least T, NK, and B cells. The use of hprt gene alterations as specific cellular markers provides a novel method for identifying stem cells in the lymphocyte lineage and for studying lymphocyte differentiation in humans.

摘要

通过使用次黄嘌呤鸟嘌呤磷酸核糖转移酶（hprt）基因改变和染色体畸变作为体内细胞标记，已成功从广岛原子弹幸存者的外周血中克隆出源自单个干细胞的人T细胞、NK细胞和B细胞。利用这些突变淋巴细胞对嘌呤类似物6-硫鸟嘌呤的抗性，对其进行了选择性克隆。克隆的淋巴细胞具有相同的hprt基因改变和相同的染色体畸变（20q-），但表现出不同的表面或功能表型以及TCR或Ig基因的不同重排。染色体畸变模式强烈表明，原始干细胞在受到原子弹辐射后开始分化为每种细胞类型。由于此处研究的人在17岁时受到原子弹轰炸，结果表明成人中至少存在T细胞、NK细胞和B细胞的共同干细胞。使用hprt基因改变作为特定细胞标记，为鉴定淋巴细胞谱系中的干细胞和研究人类淋巴细胞分化提供了一种新方法。