Bhattacharya T K, Shukla R, Chatterjee R N, Dushyanth K
ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad, India.
ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad, India.
J Biotechnol. 2017 Jan 10;241:61-68. doi: 10.1016/j.jbiotec.2016.11.012. Epub 2016 Nov 11.
Myostatin is a negative regulator of muscular growth in poultry and other animals. Of several approaches, knocking down the negative regulator is an important aspect to augment muscular growth in chicken. Knock down of myostatin gene has been performed by shRNA acting against the expression of gene in animals. Two methods of knock down of gene in chicken such as embryo manipulation and sperm mediated method have been performed. The hatching percentage in embryo manipulation and sperm mediated method of knock down was 58.0 and 41.5%, respectively. The shRNA in knock down chicken enhanced body weight at 6 weeks by 26.9%. The dressing percentage and serum biochemical parameters such as SGPT and alkaline phosphatase differed significantly (P<0.05) between knock down and control birds. It is concluded that knocking down the myostatin gene successfully augmented growth in chicken.
肌肉生长抑制素是家禽和其他动物肌肉生长的负调节因子。在多种方法中，敲除这种负调节因子是促进鸡肌肉生长的一个重要方面。在动物中，通过针对基因表达的短发夹RNA（shRNA）实现了肌肉生长抑制素基因的敲除。已经开展了两种在鸡中敲除基因的方法，即胚胎操作法和精子介导法。胚胎操作法和精子介导法敲除基因后的孵化率分别为58.0%和41.5%。敲除基因的鸡中，shRNA使6周龄时的体重增加了26.9%。敲除组和对照组鸡之间的屠宰率以及血清生化参数（如谷丙转氨酶和碱性磷酸酶）存在显著差异（P<0.05）。得出的结论是，成功敲除肌肉生长抑制素基因可促进鸡的生长。
