通过 RNAi 沉默乙酰辅酶 A 羧化酶 A 和固醇调节元件结合蛋白 1 基因可降低鸡血清和蛋胆固醇。
Silencing acetyl-CoA carboxylase A and sterol regulatory element-binding protein 1 genes through RNAi reduce serum and egg cholesterol in chicken.
机构信息
ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad, India.
出版信息
Sci Rep. 2022 Jan 24;12(1):1191. doi: 10.1038/s41598-022-05204-z.
Cholesterol is synthesized in chicken through de novo lipid biosynthetic pathway where two most important genes viz. SREBP1 and ACACA play immense role. To minimize cholesterol synthesis, RNAi approach was adopted and accordingly, we developed transgenic chicken possessing ACACA and SREBP1 shRNA constructs, which showed lower level of ACACA and SREBP1 in serum. The serum total cholesterol, triglycerides, HDL and LDL cholesterol was significantly lower by 23.8, 35.6, 26.6 and 20.9%, respectively in SREBP1 transgenic birds compared to the control. The egg total cholesterol and LDL cholesterol content was numerically lower in both ACACA and SREBP1 transgenic birds by 14.3 and 13.2%, and 10.4 and 13.7%, respectively compared to the control. It is concluded that the protocol was perfected to develop transgenic chicken through RNAi for knocking down the expression of ACACA and SREBP1 proteins, which minimized the cholesterol and triglycerides contents in serum and eggs.
胆固醇是通过从头合成脂质生物合成途径在鸡中合成的，其中两个最重要的基因 SREBP1 和 ACACA 起着重要作用。为了最大程度地减少胆固醇的合成，采用了 RNAi 方法，因此，我们开发了携带 ACACA 和 SREBP1 shRNA 构建体的转基因鸡，其血清中的 ACACA 和 SREBP1 水平较低。与对照组相比，SREBP1 转基因鸡的血清总胆固醇、甘油三酯、HDL 和 LDL 胆固醇分别显著降低 23.8%、35.6%、26.6%和 20.9%。ACACA 和 SREBP1 转基因鸡的卵总胆固醇和 LDL 胆固醇含量也分别比对照组低 14.3%和 13.2%，10.4%和 13.7%。结论是，完善了通过 RNAi 开发转基因鸡的方案，以敲低 ACACA 和 SREBP1 蛋白的表达，从而降低血清和卵中的胆固醇和甘油三酯含量。
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