Instituto de Nanociencia de Aragón (INA), Universidad de Zaragoza, C/Mariano Esquillor S/N, CP 50018, Zaragoza, Spain.
Instituto de Nanociencia de Aragón (INA), Universidad de Zaragoza, C/Mariano Esquillor S/N, CP 50018, Zaragoza, Spain; Departamento de Física de la Materia Condensada, Facultad de Ciencias, C/ Pedro Cerbuna 12, 50009, Zaragoza, Spain.
Biomaterials. 2017 Jan;114:62-70. doi: 10.1016/j.biomaterials.2016.11.008. Epub 2016 Nov 9.
Magnetic hyperthermia is a new type of cancer treatment designed for overcoming resistance to chemotherapy during the treatment of solid, inaccessible human tumors. The main challenge of this technology is increasing the local tumoral temperature with minimal side effects on the surrounding healthy tissue. This work consists of an in vitro study that compared the effect of hyperthermia in response to the application of exogenous heating (EHT) sources with the corresponding effect produced by magnetic hyperthermia (MHT) at the same target temperatures. Human neuroblastoma SH-SY5Y cells were loaded with magnetic nanoparticles (MNPs) and packed into dense pellets to generate an environment that is crudely similar to that expected in solid micro-tumors, and the above-mentioned protocols were applied to these cells. These experiments showed that for the same target temperatures, MHT induces a decrease in cell viability that is larger than the corresponding EHT, up to a maximum difference of approximately 45% at T = 46 °C. An analysis of the data in terms of temperature efficiency demonstrated that MHT requires an average temperature that is 6 °C lower than that required with EHT to produce a similar cytotoxic effect. An analysis of electron microscopy images of the cells after the EHT and MHT treatments indicated that the enhanced effectiveness observed with MHT is associated with local cell destruction triggered by the magnetic nano-heaters. The present study is an essential step toward the development of innovative adjuvant anti-cancer therapies based on local hyperthermia treatments using magnetic particles as nano-heaters.
磁热疗是一种新型的癌症治疗方法，旨在克服实体、不可接近的人类肿瘤化疗治疗中的耐药性。该技术的主要挑战是在对周围健康组织的副作用最小的情况下，提高局部肿瘤温度。这项工作包括一项体外研究，比较了在外源加热 (EHT) 源作用下的热疗效果与在相同目标温度下产生的磁热疗 (MHT) 的相应效果。将人神经母细胞瘤 SH-SY5Y 细胞负载到磁性纳米粒子 (MNP) 中，并将其包装成致密的小球，以产生与固体微肿瘤中预期的环境大致相似的环境，然后将上述方案应用于这些细胞。这些实验表明，对于相同的目标温度，MHT 诱导的细胞活力下降大于相应的 EHT，在 T = 46°C 时最大差异约为 45%。根据温度效率对数据进行分析表明，MHT 需要比 EHT 低 6°C 的平均温度才能产生类似的细胞毒性效应。对 EHT 和 MHT 处理后的细胞电子显微镜图像进行分析表明，MHT 观察到的增强效果与由磁性纳米加热器引发的局部细胞破坏有关。本研究是朝着开发基于磁性颗粒作为纳米加热器的局部热疗的创新辅助抗癌疗法迈出的重要一步。
