Wickramaratne M Nirmali, Punchihewa J C, Wickramaratne D B M
Department of Physical Sciences and Technology, Faculty of Applied Sciences, Sabaragamuwa University of Sri Lanka, Belihuloya, Sri Lanka.
Department of Pharmacy Faculty of Allied Health Sciences, University of Peradeniya, Peradeniya, Sri Lanka.
BMC Complement Altern Med. 2016 Nov 15;16(1):466. doi: 10.1186/s12906-016-1452-y.
Diabetes has caused a major burden to the health sector in the developing countries and has shown an increasing trend among the urban population. It is estimated that most patients are with type II diabetes which could be easily treated with dietary changes, exercise, and medication. Sri Lanka carries a long history ayurvedic medicine where it uses the plant for treating many diseases. Therefore it is important to screen medicinal plants scientifically so they could be used safely and effectively in the traditional medical system and also be used for further investigations. Adenanthera pavonina is a plant used in the Ayurvedic medical system in Sri Lanka for treating many diseases including diabetics. We evaluated the anti-diabetic properties and the antioxidant properties of Adenanthera pavonina leaves.
The methanol extract of the leaves was sequentially extracted with petroleum ether and thereafter was partitioned between EtOAc, and water. The α-amylase inhibition assay was performed using the 3,5- dinitrosalicylic acid method. The antioxidant activities were measured using the DPPH free radical scavenging activity and the total phenolic content using Folin-Ciocalteu's reagent. The cytotoxicity of the extract was evaluated using the Brine shrimp bioassay.
The IC values of α amylase inhibitory activity of MeOH, EtOAc, petroleum ether, and water were 16.16 ± 2.23, 59.93 ± 0.25, 145.49 ± 4.86 and 214.85 ± 9.72 μg/ml respectively and was similar to that of Acarbose (18.63 ± 1.21 (μg/ml). Antioxidant activities were also determined and the EtOAc fraction showed the highest total phenolic content (34. 62 ± 1.14 mg/g extract) and the highest DPPH scavenging activity with an IC of 249.92 ± 3.35 μg/ml.
The leaf extracts of Adenanthera pavonina exhibit remarkable α-amylase inhibitory activity in the crude methanolic extract. Hence leaves of Adenanthera pavonina has a potential to be used as a regular green vegetable and also be investigated further in isolating pure compounds with anti-diabetic activity.
糖尿病给发展中国家的卫生部门带来了沉重负担，且在城市人口中呈上升趋势。据估计，大多数患者患的是II型糖尿病，可通过饮食改变、运动和药物轻松治疗。斯里兰卡有着悠久的阿育吠陀医学历史，在该医学中使用植物治疗多种疾病。因此，科学筛选药用植物很重要，这样它们就能在传统医学体系中安全有效地使用，也可用于进一步研究。鸡冠刺桐是斯里兰卡阿育吠陀医学体系中用于治疗包括糖尿病在内多种疾病的一种植物。我们评估了鸡冠刺桐叶的抗糖尿病特性和抗氧化特性。
叶的甲醇提取物先用石油醚依次萃取，然后在乙酸乙酯和水之间进行分配。采用3,5 - 二硝基水杨酸法进行α -淀粉酶抑制试验。使用DPPH自由基清除活性测定抗氧化活性，使用福林 - 西奥尔特试剂测定总酚含量。采用卤虫生物测定法评估提取物的细胞毒性。
甲醇、乙酸乙酯、石油醚和水提取物的α -淀粉酶抑制活性的IC值分别为16.16±2.23、59.93±0.25、145.49±4.86和214.85±9.72μg/ml，与阿卡波糖（18.63±1.21（μg/ml）相似。还测定了抗氧化活性，乙酸乙酯部分显示出最高的总酚含量（34.62±1.14mg/g提取物）和最高的DPPH清除活性，IC为249.92±3.35μg/ml。
鸡冠刺桐叶提取物在粗甲醇提取物中表现出显著的α -淀粉酶抑制活性。因此，鸡冠刺桐叶有潜力用作常规绿色蔬菜，也可进一步研究分离具有抗糖尿病活性的纯化合物。
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