Florentin I, Maral J, de Sousa M, Berardet M, Hertz F, Cloarec A
Institut de Cancérologie et d'Immunogénétique (UA CNRS 1163, Association Claude Bernard), Hôpital Paul Brousse, Villejuif, France.
Int J Immunopharmacol. 1989;11(2):173-83. doi: 10.1016/0192-0561(89)90069-6.
The in vivo effects of non-steroidal anti-inflammatory drugs (NSAIDs) on the host immune system are still poorly understood. However, through inhibition of prostaglandin synthesis, NSAIDs may exhibit immunomodulating properties. The present work was aimed at evaluating the influence of niflumic acid on immune responses when administered orally for 7 consecutive days to 8-week-old inbred mice. Immunological tests were performed 24 h after the arrest of the treatment. At a dosage of 50 mg/kg/day, niflumic acid exerted noticeable immunostimulating effects, as shown by an increase in plaque-forming cell numbers after in vivo immunization with sheep red blood cells, an augmentation of spleen cell proliferation responses to stimulation with T- or B-cell mitogens and of T-cell cytotoxic response to allogenic cells. Phagocytosis-induced chemiluminescence of peritoneal macrophages was also enhanced whereas interleukin-1 production by these cells was depressed, but without concomitant modification in interleukin-2 production by T-cells. Increasing the niflumic acid dosage to 75 mg/kg resulted in the disappearance of the immunostimulatory effects on lymphocytes responses. Macrophage activities were affected similarly in mice receiving 50 mg/kg. These results demonstrate that niflumic acid is able to stimulate in vivo several immunological functions and, consequently, to maintain host immune defenses. Interestingly, it depressed interleukin-1 production, known to play a major role in the inflammatory process.
非甾体抗炎药（NSAIDs）对宿主免疫系统的体内作用仍知之甚少。然而，通过抑制前列腺素合成，NSAIDs可能具有免疫调节特性。本研究旨在评估连续7天口服尼氟灭酸对8周龄近交系小鼠免疫反应的影响。在停止给药24小时后进行免疫测试。以50毫克/千克/天的剂量给药时，尼氟灭酸表现出明显的免疫刺激作用，如用绵羊红细胞进行体内免疫后形成空斑细胞数量增加、脾细胞对T细胞或B细胞有丝分裂原刺激的增殖反应增强以及T细胞对同种异体细胞的细胞毒性反应增强。腹膜巨噬细胞吞噬诱导的化学发光也增强，而这些细胞产生的白细胞介素-1减少，但T细胞产生的白细胞介素-2没有相应改变。将尼氟灭酸剂量增加到75毫克/千克会导致对淋巴细胞反应的免疫刺激作用消失。接受50毫克/千克剂量的小鼠巨噬细胞活性也受到类似影响。这些结果表明，尼氟灭酸能够在体内刺激多种免疫功能，从而维持宿主免疫防御。有趣的是，它抑制了白细胞介素-1的产生，而白细胞介素-1在炎症过程中起主要作用。
