Ushio Y, Oda Y, Abe H
Research Institute of Oriental Medicine, Kinki University, Osaka, Japan.
Int J Immunopharmacol. 1991;13(5):501-8. doi: 10.1016/0192-0561(91)90069-j.
The in vivo effects of saikosaponin, isolated from Bupleurum radix, on the immune responses are still poorly understood. We have already shown that saikosaponin-d increases phagocytic activities of murine peritoneal macrophages such as spreading activity, phagocytosis, lysosomal enzyme activity and intracellular killing activity of living yeast. This work extends these observations by showing that treatment with saikosaponin also increased the antibody response in plaque-forming cell numbers after in vivo immunization with sheep red blood cells (SRBC) and an augmentation of spleen cell proliferation responses to stimulation with T- or B-cell mitogens both before and after immunization. Furthermore, after SRBC immunization, the macrophages from mice treated with saikosaponin-d revealed significant increases in spreading activity and lysosomal enzyme activity. The chemiluminescences of the macrophages from mice treated with saikosaponin-d stimulated by opsonized zymosan and PMA were enhanced and interleukin-1 production by the cells was increased in a dose-dependent manner. These results demonstrate that saikosaponin-d may stimulate in vivo immunological lymphocyte functions, partly by activating some macrophage functions.
从柴胡中分离出的柴胡皂苷在体内对免疫反应的影响仍知之甚少。我们已经表明，柴胡皂苷-d可增强小鼠腹腔巨噬细胞的吞噬活性，如铺展活性、吞噬作用、溶酶体酶活性以及对活酵母的细胞内杀伤活性。这项研究通过表明柴胡皂苷处理还可增加体内用绵羊红细胞（SRBC）免疫后形成空斑细胞数量的抗体反应，以及增强免疫前后脾细胞对T细胞或B细胞有丝分裂原刺激的增殖反应，扩展了这些观察结果。此外，在SRBC免疫后，用柴胡皂苷-d处理的小鼠的巨噬细胞在铺展活性和溶酶体酶活性方面有显著增加。用调理酵母聚糖和佛波酯刺激后，用柴胡皂苷-d处理的小鼠的巨噬细胞的化学发光增强，并且细胞产生白细胞介素-1呈剂量依赖性增加。这些结果表明，柴胡皂苷-d可能部分通过激活某些巨噬细胞功能来刺激体内免疫淋巴细胞功能。
