Martin T R, Galli S J, Katona I M, Drazen J M
Department of Pediatrics, Children's Hospital, Boston, Massachusetts.
J Clin Invest. 1989 Apr;83(4):1375-83. doi: 10.1172/JCI114025.
We used genetically mast cell-deficient WBB6F1-W/Wv and WCB6F1-S1/S1d mice and the congenic normal (+/+) mice to investigate the effects of intravenous infusion of goat antimouse IgE on heart rate (HR), pulmonary dynamic compliance (Cdyn), pulmonary conductance (GL), and survival. In WBB6F1-+/+ and WCB6F1-+/+ mice, anti-IgE induced extensive degranulation of tracheobronchial mast cells, as well as significant elevation of HR, significant reductions in Cdyn and GL and, in some cases, death. In contrast, W/Wv and S1/S1d mice exhibited little or no pathophysiological responses and no mortality after challenge with anti-IgE. In W/Wv mice reconstituted with mast cells by intravenous administration of bone marrow cells derived from congenic +/+ mice (+/+ BM----W/Wv mice), anti-IgE induced extensive mast cell degranulation, as well as pathophysiological responses and mortality similar to those observed in WBB6F1-+/+ mice. These findings suggest a critical role for mast cells in the development of the cardiopulmonary changes and mortality associated with anti-IgE-induced anaphylaxis.
我们使用基因性肥大细胞缺陷的WBB6F1-W/Wv和WCB6F1-S1/S1d小鼠以及同基因正常（+/+）小鼠，来研究静脉输注山羊抗小鼠IgE对心率（HR）、肺动态顺应性（Cdyn）、肺传导率（GL）和存活率的影响。在WBB6F1-+/+和WCB6F1-+/+小鼠中，抗IgE诱导气管支气管肥大细胞广泛脱颗粒，以及HR显著升高、Cdyn和GL显著降低，并且在某些情况下导致死亡。相反，W/Wv和S1/S1d小鼠在接受抗IgE攻击后表现出很少或没有病理生理反应，也没有死亡。在用来自同基因+/+小鼠的骨髓细胞静脉注射重建肥大细胞的W/Wv小鼠（+/+ BM----W/Wv小鼠）中，抗IgE诱导广泛的肥大细胞脱颗粒，以及与在WBB6F1-+/+小鼠中观察到的类似的病理生理反应和死亡率。这些发现表明肥大细胞在与抗IgE诱导的过敏反应相关的心肺变化和死亡率的发展中起关键作用。
