染色质重塑酶BRG1通过对WNT3A的正向调控促进结肠癌进展。
The chromatin-remodeling enzyme BRG1 promotes colon cancer progression via positive regulation of WNT3A.
作者信息
Lin Shengtao, Jiang Tao, Ye Ling, Han Zhongbo, Liu Yuan, Liu Chenchen, Yuan Chenwei, Zhao Senlin, Chen Jian, Wang Jingtao, Tang Huamei, Lu Su, Yang Liguang, Wang Xiaoliang, Yan Dongwang, Peng Zhihai, Fan Junwei
机构信息
Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080,China.
Department of Anal-Colorectal Surgery, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
出版信息
Oncotarget. 2016 Dec 27;7(52):86051-86063. doi: 10.18632/oncotarget.13326.
In this study, we aimed to elucidate the clinical significance and underlying mechanisms of BRG1 in colon cancer. In the clinical analysis, overexpression of BRG1 correlates with colon cancer progression in two cohorts (n = 191 and n = 75). Kaplan-Meier survival analysis revealed that BRG1 is a prognosis predictor for overall survival (P < 0.001) and disease-free survival (P = 0.001). Knocking down BRG1 expression significantly suppressed the proliferation and invasion in colon cancer cells. The expression pattern of WNT3A is consistent with BRG1 in colon cancer tissues and WNT3A expression was inhibited in BRG1 knockdown cells. In addition, restoring WNT3A expression rescues the inhibition of cell proliferation and invasion induced by BRG1. In this study, we demonstrate that BRG1 may contribute to colon cancer progression through upregulating WNT3A expression.
在本研究中，我们旨在阐明BRG1在结肠癌中的临床意义及潜在机制。在临床分析中，BRG1的过表达与两个队列（n = 191和n = 75）中的结肠癌进展相关。Kaplan-Meier生存分析显示，BRG1是总生存期（P < 0.001）和无病生存期（P = 0.001）的预后预测指标。敲低BRG1表达可显著抑制结肠癌细胞的增殖和侵袭。WNT3A在结肠癌组织中的表达模式与BRG1一致，且在BRG1敲低的细胞中WNT3A表达受到抑制。此外，恢复WNT3A表达可挽救由BRG1诱导的细胞增殖和侵袭抑制。在本研究中，我们证明BRG1可能通过上调WNT3A表达促进结肠癌进展。
Zotero 插件