Kolena B, Petrovičová I, Šidlovská M, Pilka T, Neuschlová M, Valentová I, Rybanský L', Trnovec T
1 Department of Zoology and Anthropology, Constantine the Philosopher University in Nitra, Nitra, Slovakia.
2 Department of Mathematics, Constantine the Philosopher University in Nitra, Nitra, Slovakia.
Hum Exp Toxicol. 2017 Oct;36(10):1100-1112. doi: 10.1177/0960327116678295. Epub 2016 Nov 16.
We studied occupational exposure to phthalates from first-morning-void urine sample of hairdressing apprentices by HPLC-MS/MS analyses and association with health status. Metabolites of mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono ethyl phthalate (MEP), mono- n-butyl phthalate (MnBP) and mono-iso-butyl phthalate (MiBP) were detected in all urine samples, followed by metabolites mono(2-ethyl-5-oxohexyl) phthalate (MEOHP) and mono(2-etylhexyl) phthalate (MEHP) occurring in 97.06% and 86.76% of samples, respectively. Positive associations for females were observed between MnBP and fat-free mass index (FFMI) and age; negative associations were found between MEHP, MEOHP, MEHHP, sum of MEHP, MEHHP, MEOHP and vital capacity and also between MEHP and forced vital capacity (FVC of predicted value (PV)). Lengths of exposure were associated to MnBP, MEHHP, and MEP. We also documented positive associations between anthropometry (body mass index, waist-to-height ratio (WHtR), FFMI, fat mass index) and pulmonary function FVC% of PV for females and negative associations between WHtR, waist-to-hip ratio, FFMI and ratio of forced expiratory volume in 1 s (FEV) to FVC (FEV/FVC). We assume that factors of occupational environment of hairdressing apprentices are affected by phthalates and resulted in negative outcomes in breathing mechanism and influence of body composition. Adipose tissue could play role as confounding factor in urine excretion of phthalates because of their lipid solubility and accumulation.
我们通过高效液相色谱-串联质谱（HPLC-MS/MS）分析，研究了美发学徒晨尿样本中邻苯二甲酸盐的职业暴露情况及其与健康状况的关联。在所有尿液样本中均检测到了单（2-乙基-5-羟基己基）邻苯二甲酸酯（MEHHP）、单乙基邻苯二甲酸酯（MEP）、单正丁基邻苯二甲酸酯（MnBP）和单异丁基邻苯二甲酸酯（MiBP）的代谢物，其次是单（2-乙基-5-氧代己基）邻苯二甲酸酯（MEOHP）和单（2-乙基己基）邻苯二甲酸酯（MEHP）的代谢物，分别出现在97.06%和86.76%的样本中。在女性中，观察到MnBP与去脂体重指数（FFMI）和年龄呈正相关；MEHP、MEOHP、MEHHP、MEHP、MEHHP、MEOHP总和与肺活量呈负相关，MEHP与用力肺活量（预测值（PV）的FVC）也呈负相关。暴露时长与MnBP、MEHHP和MEP相关。我们还记录了女性人体测量指标（体重指数、腰高比（WHtR）、FFMI、脂肪量指数）与肺功能PV的FVC%之间的正相关，以及WHtR、腰臀比、FFMI与1秒用力呼气量（FEV）与FVC之比（FEV/FVC）之间的负相关。我们认为，美发学徒职业环境因素受邻苯二甲酸盐影响，导致呼吸机制出现负面结果，并影响身体成分。由于邻苯二甲酸盐的脂溶性和蓄积性，脂肪组织可能在邻苯二甲酸盐的尿液排泄中起到混杂因素的作用。
