Yu Gyeong Im, Jun Sang Eun, Shin Dong Hoon
Department of Preventive Medicine, School of Medicine, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Korea.
College of Nursing, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Korea.
Inflamm Res. 2017 Mar;66(3):217-225. doi: 10.1007/s00011-016-1006-2. Epub 2016 Nov 16.
Among the inflammatory mediators involved in the pathogenesis of obesity, cell adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) stand out. The aim of this study was to investigate the associations of ICAM-1 and VCAM-1 gene variants with obesity and to investigate the associations between these genetic polymorphisms and CRP, UA, and WBC count.
Four SNPs of the VCAM-1 gene (rs3176860, rs2392221, rs3917010 and rs3176879) and two SNPs of the ICAM-1 gene (rs281432 and rs5498) were analyzed in 181 control (18 < BMI < 23) and 144 obese (BMI ≥ 25) subjects. The SNPs were genotyped by direct sequencing.
In allele frequency analysis, the G allelic frequency of rs3176860 in the VCAM-1 gene was lower in the obese group (30.9%) than in the controls (41.2%) (P = 0.007). The C allelic frequency of rs3917010 was lower in the obese group (18.1%) than in the control (25.1%) (P = 0.03). In the haplotype analysis of VCAM-1 gene, the ht1 (ACA) was higher and ht2 (GCC) was lower in the obese subjects than in the controls (P = 0.0057 and P = 0.037, respectively). In the obese group, participants carrying the G allele of rs3176860 of the VCAM-1 gene showed a higher percentage of segmented neutrophils and CRP levels than those carrying only the A allele (P = 0.028 and P = 0.042, respectively).
The results of this study suggest that VCAM-1 gene variants may be related to obesity and inflammatory markers in the Korean population.
在参与肥胖发病机制的炎症介质中，细胞黏附分子如细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）尤为突出。本研究旨在探讨ICAM-1和VCAM-1基因变异与肥胖的关联，并研究这些基因多态性与C反应蛋白（CRP）、尿酸（UA）和白细胞计数之间的关联。
对181名对照者（体重指数[BMI]为18＜BMI＜23）和144名肥胖者（BMI≥25）进行了VCAM-1基因的4个单核苷酸多态性（SNP，分别为rs3176860、rs2392221、rs3917010和rs3176879）以及ICAM-1基因的2个SNP（rs281432和rs5498）分析。通过直接测序对这些SNP进行基因分型。
在等位基因频率分析中，肥胖组中VCAM-1基因rs3176860的G等位基因频率（30.9%）低于对照组（41.2%）（P = 0.007）。肥胖组中rs3917010的C等位基因频率（18.1%）低于对照组（25.1%）（P = 0.03）。在VCAM-1基因的单倍型分析中，肥胖受试者中的单倍型1（ACA）高于对照组，单倍型2（GCC）低于对照组（分别为P = 0.0057和P = 0.037）。在肥胖组中，携带VCAM-1基因rs3176860的G等位基因的参与者的分叶核中性粒细胞百分比和CRP水平高于仅携带A等位基因的参与者（分别为P = 0.028和P = 0.042）。
本研究结果表明，在韩国人群中，VCAM-1基因变异可能与肥胖及炎症标志物有关。
