School of Life Science, Shandong University, Qingdao, 266000, China.
Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, Drug Research & Development Center, School of Pharmacy, Wannan Medical College, Wuhu, 241002, China.
Carbohydr Polym. 2019 Feb 1;205:63-71. doi: 10.1016/j.carbpol.2018.09.068. Epub 2018 Sep 29.
A novel water-soluble polysaccharide designated as TPS was isolated from the fermentation mycelia of Trichoderma kanganensis. TPS had a weight-average molecular mass of 3.074 × 10 Da, and the monosaccharide composition was consisted of Man (45.5%), GlcA (5.5%), Glc (10%), and Gal (39%). The major backbone of TPS was →6-α-d-Galp-1→5-β-d-Manf-1→5,6-β-d-Manf-1→5,6-β-d-Manf-1→, and the side chains are α-d-Glcp-1→4-α-d-Glcp-1→, β-d-Galf-1→, and α-d-Glcp-1→. In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells). TPS also showed free radical scavenging activity against hydrogen peroxide. Overall, these results suggested that TPS from Trichoderma kanganensis may have potential application in biomedical fields.
一种新型水溶性多糖 TPS 从康氏木霉发酵菌丝体中分离得到。TPS 的重均分子量为 3.074 × 10 Da，单糖组成由 Man（45.5%）、GlcA（5.5%）、Glc（10%）和 Gal（39%）组成。TPS 的主要骨架为 →6-α-d-Galp-1→5-β-d-Manf-1→5,6-β-d-Manf-1→5,6-β-d-Manf-1→，侧链为 α-d-Glcp-1→4-α-d-Glcp-1→，β-d-Galf-1→，和 α-d-Glcp-1→。此外，我们证明 TPS 在正常细胞（LO2 细胞）中没有毒性，并抑制了小鼠结肠细胞（CT26 细胞）的增殖。TPS 对过氧化氢也有清除自由基的活性。总的来说，这些结果表明康氏木霉来源的 TPS 可能在生物医学领域有潜在的应用。
