Harkness R A, Coade S B, Webster A D
Clin Chim Acta. 1984 Nov 15;143(2):91-8. doi: 10.1016/0009-8981(84)90216-x.
ATP, ADP and AMP in concentrations at least 1 mumol/l have been found by high pressure liquid chromatography (HPLC) in plasma from peripheral venous blood. Total adenine nucleotide concentrations of about 15-20 mumol/l can be found in some conventional clinical samples of blood using EDTA as an anticoagulant. EDTA prevented adenine nucleotide conversion to inosine in plasma. In order to estimate concentrations in vivo, the contribution derived from the cell breakage inherent even in careful venous blood sampling has been estimated by extrapolation to zero 'haemoglobin' concentration in plasma and minimum values in samples of small volume. Available results appear to be consistent with the release of small amounts of ATP in or near the peripheral circulation at the time of venepuncture. In CSF, ATP, ADP and AMP concentrations were less than 0.05 mumol/l suggesting that membrane activity in the central nervous system is not associated with non-specific leakage. The high Km variant of lymphocyte ecto-5'-nucleotidase was not associated with a significantly higher concentration of its substrate AMP in plasma. However, this enzyme may function on the lymphocyte in the thymus and spleen.
通过高压液相色谱法（HPLC）在外周静脉血的血浆中发现了浓度至少为1微摩尔/升的ATP、ADP和AMP。使用EDTA作为抗凝剂的一些常规临床血液样本中可发现总腺嘌呤核苷酸浓度约为15 - 20微摩尔/升。EDTA可防止血浆中腺嘌呤核苷酸转化为肌苷。为了估计体内浓度，通过外推至血浆中“血红蛋白”浓度为零以及小体积样本中的最小值，对即使在仔细采集静脉血时固有的细胞破裂所产生的贡献进行了估计。现有结果似乎与静脉穿刺时外周循环中或其附近少量ATP的释放一致。在脑脊液中，ATP、ADP和AMP浓度低于0.05微摩尔/升，这表明中枢神经系统中的膜活性与非特异性渗漏无关。淋巴细胞ecto - 5'-核苷酸酶的高Km变体与血浆中其底物AMP的浓度显著升高无关。然而，这种酶可能在胸腺和脾脏中的淋巴细胞上发挥作用。
