一株澳大利亚芜菁花叶病毒分离株连续传代七年前后的全核苷酸序列
Complete Nucleotide Sequence of an Australian Isolate of Turnip mosaic virus before and after Seven Years of Serial Passaging.
作者信息
Pretorius Lara, Moyle Richard L, Dalton-Morgan Jessica, Hussein Nasser, Schenk Peer M
机构信息
Nexgen Plants Pty. Ltd. and School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Queensland, Australia.
Nexgen Plants Pty. Ltd. and School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Queensland, Australia
出版信息
Genome Announc. 2016 Nov 17;4(6):e01269-16. doi: 10.1128/genomeA.01269-16.
Abstract
The complete genome sequence of an Australian isolate of Turnip mosaic virus was determined by Sanger sequencing. After seven years of serial passaging by mechanical inoculation, the isolate was resequenced by RNA sequencing (RNA-Seq). Eighteen single nucleotide polymorphisms were identified between the isolates. Both isolates had 96% identity to isolate AUST10.
摘要
通过桑格测序法确定了一株澳大利亚芜菁花叶病毒分离株的完整基因组序列。在通过机械接种进行连续传代培养七年后，该分离株通过RNA测序（RNA-Seq）重新测序。在这些分离株之间鉴定出了18个单核苷酸多态性。这两个分离株与AUST10分离株均具有96%的同一性。
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