Zhao Bin, Zhu Zilong, Hao Junwei, Wan Zongming, Guo Xiaoqin
1 Department of Neurology, Tianjin Medical University General Hospital, Tianjin, China.
2 Department of Neurology, Tianjin Huanhu Hospital, Tianjin, China.
J Int Med Res. 2016 Dec;44(6):1331-1338. doi: 10.1177/0300060516665707. Epub 2016 Nov 18.
Objective To determine the expression and clinical significance of plasma miR-335 in patients with acute ischemic stroke (AIS) and investigate its association with calmodulin (CaM) expression. Methods Plasma miR-335 and CaM expression levels in patients with AIS and healthy controls were examined. Correlations between miR-335, CaM, and National Institutes of Health Stroke Scale scores were also analysed. Furthermore, the potential regulatory function of miR-335 on CaM expression was investigated. Results Plasma miR-335 levels were significantly lower in AIS and negatively correlated with NIHSS scores. The converse was observed for plasma CaM levels. Plasma miR-335 and CaM levels were negatively correlated. Plasma miR-335 was confirmed as a novel biomarker for AIS diagnosis and an independent predictor of AIS. Up-regulation of miR-335 suppressed CaM protein expression, and CaM was confirmed as a direct target of miR-335. Conclusions Plasma miR-335 was down-regulated in AIS patients and represents a potential noninvasive circulating biomarker.
目的 确定急性缺血性脑卒中（AIS）患者血浆miR-335的表达及临床意义，并探讨其与钙调蛋白（CaM）表达的关系。方法 检测AIS患者和健康对照者血浆中miR-335和CaM的表达水平。分析miR-335、CaM与美国国立卫生研究院卒中量表（NIHSS）评分之间的相关性。此外，研究miR-335对CaM表达的潜在调控作用。结果 AIS患者血浆miR-335水平显著降低，且与NIHSS评分呈负相关。血浆CaM水平则相反。血浆miR-335与CaM水平呈负相关。血浆miR-335被确认为AIS诊断的新型生物标志物及AIS的独立预测指标。miR-335的上调抑制了CaM蛋白表达，且CaM被确认为miR-335的直接靶点。结论 AIS患者血浆miR-335表达下调，是一种潜在的非侵入性循环生物标志物。
