Liu Yanping, Zhang Junjian, Han Rongfei, Liu Hanxing, Sun Dong, Liu Xuan
Department of Neurology, Zhongnan Hospital, Wuhan University, Donghu Road 169, Wuhan 430071, China; Department of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Department of Neurology, Zhongnan Hospital, Wuhan University, Donghu Road 169, Wuhan 430071, China.
J Clin Neurosci. 2015 Feb;22(2):291-5. doi: 10.1016/j.jocn.2014.05.042. Epub 2014 Sep 23.
Cerebral ischemic injury activates a robust inflammatory response, exacerbating neurological deficit. Several brain specific microRNA (miRNA) molecules have been reported to mediate functioning of the immune system, referred to as NeurimmiR. We aimed to explore possible associations between serum miRNA levels and stroke severity and their involvement in the regulation of inflammatory responses after stroke. Blood samples were obtained from 31 patients with acute ischemic stroke and 11 healthy controls. We evaluated infarct volume using diffusion weighted imaging and neurological deficit using the National Institutes of Health Stroke Scale. Serum levels of three NeurimmiR, miR-124, miR-9 and miR-219 were detected by real-time polymerase chain reaction and serum levels of metalloproteinase-9 (MMP-9), a proinflammation marker in brain injury, were examined by enzyme-linked immunosorbent assay. We found that serum miR-124 was significantly decreased within 24 hours after stroke onset and serum miR-9 was decreased in patients with larger stroke. There were no significant changes in serum miR-219. Both serum miR-124 and miR-9 levels within 24 hours were negatively correlated with infarct volume and plasma high-sensitivity C-reactive protein levels. All three NeurimmiR negatively correlated with MMP-9 levels. Our preliminary findings indicate that serum miR-124, miR-9 and miR-219 are suppressed in acute ischemic stroke thus facilitating neuroinflammation and brain injury.
脑缺血损伤会引发强烈的炎症反应，加剧神经功能缺损。已有报道称，几种脑特异性微小RNA（miRNA）分子可介导免疫系统的功能，称为神经miRNA（NeurimmiR）。我们旨在探讨血清miRNA水平与中风严重程度之间的可能关联，以及它们在中风后炎症反应调节中的作用。从31例急性缺血性中风患者和11名健康对照者中采集血样。我们使用弥散加权成像评估梗死体积，并使用美国国立卫生研究院卒中量表评估神经功能缺损。通过实时聚合酶链反应检测三种神经miRNA（miR-124、miR-9和miR-219）的血清水平，并通过酶联免疫吸附测定法检测脑损伤中的促炎标志物金属蛋白酶-9（MMP-9）的血清水平。我们发现，中风发作后24小时内血清miR-124显著降低，中风较大的患者血清miR-9降低。血清miR-219无显著变化。中风后24小时内血清miR-124和miR-9水平均与梗死体积和血浆高敏C反应蛋白水平呈负相关。所有三种神经miRNA均与MMP-9水平呈负相关。我们的初步研究结果表明，急性缺血性中风中血清miR-124、miR-9和miR-219受到抑制，从而促进神经炎症和脑损伤。
