Bouillot M, Choppin J, Cornille F, Martinon F, Papo T, Gomard E, Fournie-Zaluski M C, Levy J P
Laboratoire d'Immunologie et d'Oncologie des Maladies Rétrovirales, INSERM U 152, CNRS UA 628, Hôpital Cochín, Paris, France.
Nature. 1989 Jun 8;339(6224):473-5. doi: 10.1038/339473a0.
Antigenic peptides are presented to T lymphocytes by major histocompatibility complex (MHC) molecules. The binding of peptides to MHC class II molecules has been demonstrated directly, and is found to correlate with the ability of specific class II alleles to restrict the T-cell response to specific peptides. By comparison, a direct demonstration of a physical association between antigenic peptides and MHC class I molecules has proved difficult. A recent report shows that it is possible, however, and the three-dimensional structure of a class I MHC molecule illustrates the site where such binding must occur. Here we describe a simple assay which measures the binding of radiolabelled MHC class I molecules to peptides bound to a solid phase support. We find that class I molecules bind specifically to peptides known to be antigenic for class I-restricted cytotoxic T lymphocytes. Peptides which are recognized by cytotoxic T lymphocytes bind not only to the restricting MHC class I molecule but also to other class I molecules. Our results suggest that quantitative differences in the peptide/MHC class I interaction may influence the-pattern of MHC restriction observed in vivo.
抗原肽由主要组织相容性复合体（MHC）分子呈递给T淋巴细胞。肽与MHC II类分子的结合已得到直接证实，并且发现其与特定II类等位基因限制T细胞对特定肽的反应能力相关。相比之下，直接证明抗原肽与MHC I类分子之间存在物理关联却很困难。然而，最近的一份报告表明这是可能的，并且I类MHC分子的三维结构说明了这种结合必定发生的位点。在此，我们描述了一种简单的检测方法，该方法可测量放射性标记的MHC I类分子与结合在固相支持物上的肽的结合情况。我们发现I类分子特异性地结合已知对I类限制性细胞毒性T淋巴细胞具有抗原性的肽。被细胞毒性T淋巴细胞识别的肽不仅与限制性MHC I类分子结合，还与其他I类分子结合。我们的结果表明，肽/MHC I类相互作用中的定量差异可能会影响体内观察到的MHC限制模式。
