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Identification of a novel site specific endonuclease produced by Mycoplasma fermentans: discovery while characterizing DNA binding proteins in T lymphocyte cell lines.

作者信息

Halden N F, Wolf J B, Leonard W J

机构信息

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.

出版信息

Nucleic Acids Res. 1989 May 11;17(9):3491-9. doi: 10.1093/nar/17.9.3491.

DOI:10.1093/nar/17.9.3491
PMID:2786191
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC317791/
Abstract

We have discovered a new restriction endonuclease, MfeI, in nuclear extracts from T cells contaminated with Mycoplasma fermentans. This endonuclease was identified while studying proteins binding to the interleukin-2 receptor alpha chain gene promoter. MfeI cuts at the recognition sequence C'AATTG generating EcoRI compatible cohesive ends. Potential applications are discussed.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/095e/317791/abfb9d277b82/nar00126-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/095e/317791/92cad6da7a37/nar00126-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/095e/317791/65a93512c50a/nar00126-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/095e/317791/a4fe36af9b5e/nar00126-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/095e/317791/abfb9d277b82/nar00126-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/095e/317791/92cad6da7a37/nar00126-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/095e/317791/65a93512c50a/nar00126-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/095e/317791/a4fe36af9b5e/nar00126-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/095e/317791/abfb9d277b82/nar00126-0171-a.jpg

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本文引用的文献

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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89. doi: 10.1093/nar/11.5.1475.
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Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.染色质中与热休克基因激活有关的两个蛋白质结合位点。
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Structure of the human interleukin-2 receptor gene.
Science. 1985 Nov 8;230(4726):633-9. doi: 10.1126/science.2996141.
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Restriction enzymes and their isoschizomers.限制性内切酶及其同裂酶。
Nucleic Acids Res. 1988;16 Suppl(Suppl):r271-313. doi: 10.1093/nar/16.suppl.r271.
8
Chromosomal subunits in active genes have an altered conformation.活跃基因中的染色体亚基具有改变的构象。
Science. 1976 Sep 3;193(4256):848-56. doi: 10.1126/science.948749.
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DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.DNA酶足迹法：一种检测蛋白质与DNA结合特异性的简单方法。
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