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Characterization of restriction endonuclease AjoI from Acinetobacter johnsonii.

作者信息

Nowak A, Kur J, Gospodarek E, Bielawski K

机构信息

Department of Microbiology, Technical University of Gdańsk, Poland.

出版信息

FEMS Microbiol Lett. 1994 Mar 15;117(1):97-102. doi: 10.1111/j.1574-6968.1994.tb06748.x.

Abstract

A new type II restriction endonuclease, named AjoI, was detected in Acinetobacter johnsonii. The enzyme AjoI, an isoschizomer of PstI, recognized the hexanucleotide sequence [5'-CTGCA/G-3'], with a cleavage site generating fragments of DNA with protruding cohesive 3' termini.

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