Zhang Yupan, Wang Haifeng, Lou Xiaoqian, Qu Xiaozhang, Gao Lichao, Liu Xiaolei, Li Man, Guo Hui, Jiang Yanfang
Department of Central Laboratory, The First Hospital, Jilin University, Changchun, China.
Department of Endocrinology, Weihai Municipal Hospital, Weihai, China.
Clin Exp Pharmacol Physiol. 2017 Feb;44(2):180-190. doi: 10.1111/1440-1681.12699.
Type 1 diabetes mellitus (T1DM) is characterized by absolute insulin deficiency owing to autoimmune destruction of the pancreatic β cells. A significant decrease in natural killer (NK) cells in peripheral blood has been observed in patients with untreated T1DM. In the present study, we aimed to explore the role of NK cells and their subsets in young T1DM patients. A total of 30 children and adolescents with untreated T1DM and 27 healthy controls (HC) were recruited in this study. Flow cytometry analysis indicated that the percentage of peripheral blood CD3-CD56+ NK cells and NK cells subsets (CD56bright, CD56dim and CD56neg), were significantly decreased in the T1DM patients compared to healthy controls. In addition, the percentage of inducible CD107a+ and IFN-γ-secreting NK cells was significantly decreased compared to HC. Interestingly, the percentage of NKG2D+ NK cells negatively correlated with the level of serum TCHOL and TG in T1DM patients. Our data indicate that decreased number and impaired function of NK cells may have a role in the pathogenesis of T1DM.
1型糖尿病（T1DM）的特征是由于胰腺β细胞的自身免疫性破坏导致绝对胰岛素缺乏。在未经治疗的T1DM患者中，已观察到外周血中自然杀伤（NK）细胞显著减少。在本研究中，我们旨在探讨NK细胞及其亚群在年轻T1DM患者中的作用。本研究共招募了30名未经治疗的儿童和青少年T1DM患者以及27名健康对照（HC）。流式细胞术分析表明，与健康对照相比，T1DM患者外周血CD3-CD56+NK细胞及其亚群（CD56bright、CD56dim和CD56neg）的百分比显著降低。此外，与HC相比，可诱导的CD107a+和分泌IFN-γ的NK细胞百分比显著降低。有趣的是，T1DM患者中NKG2D+NK细胞的百分比与血清总胆固醇（TCHOL）和甘油三酯（TG）水平呈负相关。我们的数据表明，NK细胞数量减少和功能受损可能在T1DM的发病机制中起作用。
