Liu Yuzhou, Wang Le, Meng Chong, Zhou Yingjie, Lao Jie, Zhao Xin
Department of Hand Surgery, Huashan Hospital, Fudan University, 12 Wulumuqi Zhong Road, Shanghai 200040, China.
Department of Hand Surgery, Huashan Hospital, Fudan University, 12 Wulumuqi Zhong Road, Shanghai 200040, China; Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai, China; Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai, China.
Injury. 2017 Feb;48(2):253-261. doi: 10.1016/j.injury.2016.11.007. Epub 2016 Nov 9.
The study was to introduce a new and reliable behavioral model of upper trunk of brachial plexus avulsion for the study of persistent neuropathic pain. 60 rats were divided into three groups randomly: upper trunk of brachial plexus avulsion (UTBPA) group (20), global brachial plexus avulsion (GBPA) group (20), and sham- operated group (20). The animals were tested for behavioral responsiveness before surgeries and 3, 7, 14, 21, 28, 56, 84days after surgeries. The injured level of spinal cord was resected and the sections were processed for GFAP (astrocyte) and Iba1 (microglia) immunohistochemistry 3 weeks after surgeries. The UTBPA group developed significant signs both of mechanical and cold hypersensitivity, which matched the immunohistochemistry result, as well as the nature of avulsion was close to the clinical type of injury, the UTBPA group could be used as a suitable and effective persistent neuropathic pain model following brachial plexus injury.
本研究旨在引入一种新的、可靠的臂丛上干撕脱伤行为模型，用于持续性神经性疼痛的研究。将60只大鼠随机分为三组：臂丛上干撕脱伤（UTBPA）组（20只）、全臂丛撕脱伤（GBPA）组（20只）和假手术组（20只）。在手术前以及手术后3、7、14、21、28、56、84天对动物进行行为反应测试。术后3周切除脊髓损伤节段并进行GFAP（星形胶质细胞）和Iba1（小胶质细胞）免疫组织化学处理。UTBPA组出现了明显的机械性和冷超敏反应体征，这与免疫组织化学结果相符，且撕脱伤的性质与临床损伤类型相近，UTBPA组可作为臂丛神经损伤后合适且有效的持续性神经性疼痛模型。
