Hsieh Yueh-Ling, Lu Yu-Lin, Yang Nian-Pu, Yang Chen-Chia
Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung 40402, Taiwan.
Department of Physical Medicine and Rehabilitation, Hsin-Chu Branch, National Taiwan University Hospital, Hsinchu 30068, Taiwan.
Int J Mol Sci. 2023 Sep 8;24(18):13856. doi: 10.3390/ijms241813856.
This study evaluated and compared the functional recovery and histopathological outcomes of treatment involving low-intensity pulsed ultrasound (LIPUS) and methylcobalamin (B12) on brachial plexus injury (BPI) in an experimental rat model. Three days after BPI, the rats were assigned to receive either LIPUS or methylcobalamin alone or in combination consecutively for 12 days. Serial changes in sensory and motor behavioral responses, as well as morphological and immunohistochemical changes for substance P (SP), ionized calcium-binding adapter molecule 1 (iba1), brain-derived neurotrophic factor (BDNF), and S100 were examined 28 days after BPI as the outcome measurements. Early intervention of LIPUS and methylcobalamin, whether alone or in combination, augmented the sensory and motor behavioral recovery as well as modulated SP and iba1 expression in spinal dorsal horns, BDNF, and S100 in the injured nerve. Moreover, the combined therapy with its synergistic effect gave the most beneficial effect in accelerating functional recovery. In view of the effective initiation of early recovery of sensory and motor functions, treatment with LIPUS and methylcobalamin in combination has a potential role in the clinical management of early-phase BPI.
本研究评估并比较了低强度脉冲超声（LIPUS）和甲钴胺（维生素B12）对实验性大鼠模型臂丛神经损伤（BPI）的治疗效果，包括功能恢复和组织病理学结果。臂丛神经损伤后3天，将大鼠连续12天分别单独接受LIPUS或甲钴胺治疗，或两者联合治疗。在臂丛神经损伤28天后，检测感觉和运动行为反应的系列变化，以及P物质（SP）、离子钙结合衔接分子1（iba1）、脑源性神经营养因子（BDNF）和S100的形态学和免疫组化变化，作为结果测量指标。LIPUS和甲钴胺的早期干预，无论单独使用还是联合使用，均能增强感觉和运动行为恢复，并调节脊髓背角中SP和iba1的表达、损伤神经中BDNF和S100的表达。此外，联合治疗及其协同作用在加速功能恢复方面产生了最有益的效果。鉴于感觉和运动功能早期恢复的有效启动，LIPUS和甲钴胺联合治疗在臂丛神经损伤早期的临床管理中具有潜在作用。
