Tirado Yanely, Puig Alina, Alvarez Nadine, Borrero Reinier, Aguilar Alicia, Camacho Frank, Reyes Fatima, Fernandez Sonsire, Perez Jose Luis, Acevedo Reynaldo, Mata Espinoza Dulce, Payan Jorge Alberto Barrios, Garcia Maria de Los A, Kadir Ramlah, Sarmiento María E, Hernandez-Pando Rogelio, Norazmi Mohd-Nor, Acosta Armando
Institute Finlay, La Habana, Cuba.
Experimental Pathology Section, Department of Pathology, National Institute of Medical Sciences and Nutrition "Salvador Zubiran", D.F. Mexico, Mexico.
Tuberculosis (Edinb). 2016 Dec;101:44-48. doi: 10.1016/j.tube.2016.07.017. Epub 2016 Aug 5.
Tuberculosis (TB) remains an important cause of mortality and morbidity. The TB vaccine, BCG, is not fully protective against the adult form of the disease and is unable to prevent its transmission although it is still useful against severe childhood TB. Hence, the search for new vaccines is of great interest. In a previous study, we have shown that proteoliposomes obtained from Mycobacterium smegmatis (PLMs) induced cross reactive humoral and cellular response against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLMs, a murine model of progressive pulmonary TB was used. Animals immunized with PLMs with and without alum (PLMs/PLMsAL respectively) showed protection compared to non-immunized animals. Mice immunized with PLMsAL induced similar protection as that of BCG. Animals immunized with BCG, PLMs and PLMsAL showed a significant decrease in tissue damage (percentage of pneumonic area/lung) compared to non-immunized animals, with a more prominent effect in BCG vaccinated mice. The protective effect of the administration of PLMs in mice supports its future evaluation as experimental vaccine candidate against Mtb.
结核病（TB）仍然是导致死亡和发病的重要原因。卡介苗（BCG）作为结核病疫苗，虽然对预防儿童重症结核病仍有作用，但对成人结核病的防护并不完全，也无法阻止其传播。因此，研发新型疫苗备受关注。在之前的一项研究中，我们发现从耻垢分枝杆菌获得的蛋白脂质体（PLMs）可诱导针对结核分枝杆菌（Mtb）抗原的交叉反应性体液免疫和细胞免疫。为评估PLMs的保护能力，我们使用了进行性肺结核小鼠模型。与未免疫的动物相比，用含和不含明矾的PLMs（分别为PLMs/PLMsAL）免疫的动物表现出了保护作用。用PLMsAL免疫的小鼠诱导出了与卡介苗相似的保护效果。与未免疫的动物相比，用卡介苗、PLMs和PLMsAL免疫的动物组织损伤（肺内肺炎面积百分比）显著降低，其中卡介苗接种小鼠的效果更为显著。在小鼠中给予PLMs的保护作用支持其作为抗Mtb实验性候选疫苗进行进一步评估。
