Luo Xu Min, Liu Xin Yan, Tang Ji Hong, Yang Wei, Ni Zhen Hua, Chen Qing Ge, Wang Xiongbiao
Department of Respiratory Medicine, Putuo Hospital Affilated Shanghai University of Traditional Chinese Medicine , Shanghai, China.
Clin Transl Immunology. 2016 Oct 7;5(10):e103. doi: 10.1038/cti.2016.57. eCollection 2016 Oct.
Chronic obstructive pulmonary disease (COPD) is an inflammation disorder and possibly an autoimmune disease. The components of the autoimmune response in the circulatory system are of considerable interest to clinicians. Because aberrations of costimulation status have been noted in COPD, the presence of autoantibodies to B7 costimulatory factor CD80 were investigated in a cohort of patients. Recombinant rs1CD80 (lacking the transmembrane domain of CD80) was used for Western blot analysis and ELISA to investigate the presence of autoantibodies in sera of patients with stable COPD and in controls without COPD. Cytokines IL-6 and IL-8 were detected using ELISA. Western blot revealed a specific band reacting to rs1CD80 by diluting sera pool of patients, which indicated the existence of autoantibodies to CD80. The serum level of anti-rs1CD80 was higher in patients with COPD than in controls(=0.0185) and was positively correlated to the serum level of IL-6 (=0.797, <0.001) and IL-8 (=0.608, <0.001). There was a tendency that more higher level of anti-rs1CD80, more severe COPD stage. The existence of autoantibodies to costimulatory factor CD80 may suggest a pathogenic role of costimulatory factors in COPD.
慢性阻塞性肺疾病（COPD）是一种炎症性疾病，也可能是自身免疫性疾病。循环系统中自身免疫反应的组成部分引起了临床医生的极大兴趣。由于在COPD中已注意到共刺激状态异常，因此在一组患者中研究了针对B7共刺激因子CD80的自身抗体的存在情况。使用重组rs1CD80（缺乏CD80的跨膜结构域）进行蛋白质印迹分析和酶联免疫吸附测定（ELISA），以研究稳定期COPD患者和无COPD对照者血清中自身抗体的存在情况。使用ELISA检测细胞因子白细胞介素-6（IL-6）和白细胞介素-8（IL-8）。蛋白质印迹显示，通过稀释患者血清池可出现一条与rs1CD80反应的特异性条带，这表明存在针对CD80的自身抗体。COPD患者血清中抗rs1CD80水平高于对照组（=0.0185），且与IL-6血清水平呈正相关（=0.797，<0.001）以及与IL-8血清水平呈正相关（=0.608，<0.001）。存在一种趋势，即抗rs1CD80水平越高，COPD阶段越严重。共刺激因子CD80自身抗体的存在可能表明共刺激因子在COPD中具有致病作用。
