Moriguchi S, Toba M, Kishino Y
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
J Nutr Sci Vitaminol (Tokyo). 1989 Feb;35(1):49-59. doi: 10.3177/jnsv.35.49.
Phagocytosis of opsonized sheep red blood cells (SRBC) by alveolar macrophages (AM) was measured in rats fasted for 1 to 9 days or fed on diets restricted 20 to 95% compared to control group for 2 and 8 weeks. In rats fasted for 1 to 6 days, AM showed an increased phagocytosis at 2 days after fasting, but their phagocytic activity remarkably decreased afterwards. Furthermore, phagocytic activity of AM per rat revealed much more decrease at 3 to 6 days after fasting. Then the production of interleukin-1 (IL-1) by AM increased with prolonged fasting, but the production of prostaglandin E2 (PGE2) by AM cultured with lipopolysaccharide (LPS) conversely decreased in rats fasted for 2 days or longer. The proliferation of splenocytes increased with prolonged fasting. On the other hand, 20 to 95% restricted diets induced the increased phagocytosis of AM with prolonged experimental period. However, phagocytic activity of AM per rat showed significant increase only in rats on a 40% restricted diet. The findings suggest that differences in both duration and degree of dietary restriction modulate phagocytic function of AM, and may contribute to explaining, in part, conflicting observations which have been obtained on the immunologic state in malnourished animals.
在禁食1至9天的大鼠或与对照组相比饮食限制20%至95%达2周和8周的大鼠中，测定了肺泡巨噬细胞（AM）对调理过的绵羊红细胞（SRBC）的吞噬作用。在禁食1至6天的大鼠中，AM在禁食2天后吞噬作用增强，但随后其吞噬活性显著降低。此外，每只大鼠的AM吞噬活性在禁食后3至6天下降得更多。然后，随着禁食时间延长，AM产生的白细胞介素-1（IL-1）增加，但在禁食2天或更长时间的大鼠中，用脂多糖（LPS）培养的AM产生的前列腺素E2（PGE2）反而减少。脾细胞的增殖随着禁食时间延长而增加。另一方面，20%至95%的限制饮食随着实验时间延长诱导AM的吞噬作用增强。然而，仅在饮食限制40%的大鼠中，每只大鼠的AM吞噬活性显示出显著增加。这些发现表明，饮食限制的持续时间和程度的差异调节了AM的吞噬功能，并且可能部分有助于解释在营养不良动物免疫状态方面所获得的相互矛盾的观察结果。
