Nii Y, Moriguchi S, Kishino Y
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
J Nutr Sci Vitaminol (Tokyo). 1992 Dec;38(6):565-78. doi: 10.3177/jnsv.38.565.
Phagocytosis of rat alveolar macrophages (AM) was enhanced by the infusion of arginine-rich solution for 7 days. The enhancement of phagocytosis by arginine-rich solution was due to not the difference in the distribution of AM subpopulations (I to IV) but the difference in phagocytic activity of AM in fraction IV. In the process of phagocytosis, there were no significant differences in the stages of migration, attachment, and digestion between control and arginine-rich solutions, although AM from fraction IV of rats infused with arginine-rich solution showed significantly higher ingestion of opsonized sheep red blood cells (SRBC) compared to that of control group. Furthermore, the production of macrophage-activating factor (MAF) from rat splenocytes was higher in arginine-rich group than that of control group. AM from fraction IV of rats fed a stock diet had a higher arginase activity and showed a significant increase of phagocytosis following in vitro incubation with L-arginine (25 and 50 mM) for 24 h. From these results, the enhanced phagocytosis of AM by arginine-rich solution may be due to the increased phagocytosis of AM from fraction IV, in which the higher sensitivity of AM from fraction IV to arginine and the higher production of MAF from splenocytes following the infusion of arginine-rich solution participate.
连续7天输注富含精氨酸的溶液可增强大鼠肺泡巨噬细胞（AM）的吞噬作用。富含精氨酸的溶液对吞噬作用的增强并非源于AM亚群（I至IV）分布的差异，而是由于IV组中AM吞噬活性的差异。在吞噬过程中，对照组和富含精氨酸溶液组在迁移、附着和消化阶段没有显著差异，尽管输注富含精氨酸溶液的大鼠IV组中的AM与对照组相比，对调理的绵羊红细胞（SRBC）的摄取显著更高。此外，富含精氨酸组大鼠脾细胞产生的巨噬细胞激活因子（MAF）高于对照组。喂食普通饲料的大鼠IV组中的AM具有较高的精氨酸酶活性，并且在与L-精氨酸（25和50 mM）体外孵育24小时后吞噬作用显著增加。从这些结果来看，富含精氨酸的溶液增强AM的吞噬作用可能是由于IV组中AM吞噬作用的增加，其中IV组中的AM对精氨酸更高的敏感性以及输注富含精氨酸溶液后脾细胞产生更高水平的MAF都参与其中。
