Moriguchi S, Werner L, Watson R R
Immunology. 1985 Sep;56(1):169-77.
High dietary intakes (4000-650,000 IU/kg diet) of vitamin A (retinyl palmitate, RP) modified the functions of peritoneal macrophages (PM). The number of peritoneal exudated cells (PEC) obtained from CD-1 mice increased significantly at both 7 and 10 weeks after initiation of the RP diets. The percentage of PM in PEC showed no significant difference between dietary groups and was at levels of 55-60%. PM from mice fed high RP diets showed higher tumoricidal activities than PM from controls without any preincubation with macrophage activators. Enhancement of in vitro tumoricidal activity of PM increased with increasing contents of RP in the diets, reaching 30% lysis by PM isolated from mice fed the highest RP (650,000 IU/kg diet) diet. However, the in vitro activation of tumoricidal ability of PM by macrophage-activating factor (MAF) was inversely correlated with the dietary RP content. The tumoricidal activities of PM from mice fed the highest RP diet were not enhanced by MAF. However, these PM showed an increased ability to phagocytose SRBC and opsonized SRBC compared to controls. Splenocytes and thymocytes were incubated with [3H]thymidine immediately after isolation and their mitogenic activities were measured. Splenocytes, but not thymocytes, isolated from mice fed the highest RP diet had increased mitogenesis. On the other hand, NK activity was not affected by dietary RP intake. There was a similar lysis of target cells by both splenocytes and thymocytes from mice fed diets with various RP levels. IL-1 was produced from PM by incubation with LPS, and its production was assessed using the proliferation of normal mice thymocytes. Production of IL-1 in vitro showed about a two-fold increase using cells from mice fed the highest RP diet compared to controls. High RP diets induced increased phagocytic ability and tumoricidal activity of PM but did not enhance NK activity. These findings suggest that high RP diet may cause activation of PM.
高膳食维生素A（棕榈酸视黄酯，RP）摄入量（4000 - 650,000 IU/kg日粮）改变了腹膜巨噬细胞（PM）的功能。从CD - 1小鼠获得的腹膜渗出细胞（PEC）数量在开始RP日粮后的第7周和第10周均显著增加。日粮组之间PEC中PM的百分比无显著差异，处于55 - 60%的水平。喂食高RP日粮小鼠的PM显示出比未用巨噬细胞激活剂预孵育的对照组更高的杀肿瘤活性。PM的体外杀肿瘤活性增强随着日粮中RP含量的增加而增加，从喂食最高RP（650,000 IU/kg日粮）日粮的小鼠分离的PM达到30%的裂解率。然而，巨噬细胞激活因子（MAF）对PM杀肿瘤能力的体外激活与日粮RP含量呈负相关。喂食最高RP日粮小鼠的PM的杀肿瘤活性未被MAF增强。然而，与对照组相比，这些PM显示出吞噬绵羊红细胞（SRBC）和调理SRBC的能力增强。脾细胞和胸腺细胞在分离后立即与[3H]胸腺嘧啶核苷一起孵育，并测量它们的促有丝分裂活性。从喂食最高RP日粮的小鼠分离的脾细胞（而非胸腺细胞）有增加的有丝分裂。另一方面，NK活性不受日粮RP摄入量的影响。喂食不同RP水平日粮的小鼠的脾细胞和胸腺细胞对靶细胞的裂解相似。通过与脂多糖（LPS）孵育从PM产生白细胞介素 - 1（IL - 1），并使用正常小鼠胸腺细胞的增殖来评估其产生。与对照组相比，使用来自喂食最高RP日粮小鼠的细胞，体外IL - 1的产生显示出约两倍的增加。高RP日粮诱导PM的吞噬能力和杀肿瘤活性增加，但未增强NK活性。这些发现表明高RP日粮可能导致PM的激活。
