嗜肺军团菌在小鼠腹腔巨噬细胞培养物中诱导白细胞介素1的产生。

Induction of interleukin 1 by Legionella pneumophila in murine peritoneal macrophage cultures.

作者信息

Widen R H, Klein T W, Newton C A, Friedman H

机构信息

University of South Florida College of Medicine, Tampa.

出版信息

Proc Soc Exp Biol Med. 1989 Jul;191(3):304-8. doi: 10.3181/00379727-191-42925.

DOI:10.3181/00379727-191-42925

PMID:2787029

Abstract

Legionella pneumophila-induced production of both membrane-associated and secreted interleukin 1 (mIL-1 and sIL-1, respectively) was examined utilizing peritoneal macrophages from BALB/c mice. The Legionella preparations for these studies included viable bacteria and formalin-killed whole cell preparations. Both of the preparations induced mIL-1 and sIL-1 in a dose-dependent fashion. However, the viable bacteria required about 1 log lower concentrations than the formalin-killed bacteria to induce the same level of IL-1 activity measured in the thymocyte proliferation assay. Kinetic studies showed that mIL-1 and sIL-1 were detectable within 4 hr after addition of either of the L. pneumophila preparations to the peritoneal macrophage cultures, with peak levels achieved within 24 hr. These results indicate that L. pneumophila is a potent inducer of both mIL-1 and sIL-1 in normal mouse peritoneal macrophage cultures.

摘要

利用BALB/c小鼠的腹腔巨噬细胞，检测了嗜肺军团菌诱导产生的膜相关白细胞介素1（mIL-1）和分泌型白细胞介素1（sIL-1）。这些研究中使用的军团菌制剂包括活细菌和福尔马林灭活的全细胞制剂。两种制剂均以剂量依赖方式诱导mIL-1和sIL-1。然而，活细菌诱导相同水平的白细胞介素1活性（通过胸腺细胞增殖试验测定）所需的浓度比福尔马林灭活细菌低约1个对数级。动力学研究表明，将任何一种嗜肺军团菌制剂添加到腹腔巨噬细胞培养物中后4小时内即可检测到mIL-1和sIL-1，24小时内达到峰值水平。这些结果表明，在正常小鼠腹腔巨噬细胞培养物中，嗜肺军团菌是mIL-1和sIL-1的有效诱导剂。