中国中部和东南部绵羊和山羊中的嗜吞噬细胞无形体
Anaplasma phagocytophilum in sheep and goats in central and southeastern China.
作者信息
Yang Jifei, Liu Zhijie, Niu Qingli, Liu Junlong, Han Rong, Guan Guiquan, Li Youquan, Liu Guangyuan, Luo Jianxun, Yin Hong
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, People's Republic of China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China.
出版信息
Parasit Vectors. 2016 Nov 21;9(1):593. doi: 10.1186/s13071-016-1880-z.
BACKGROUND
Anaplasma phagocytophilum is wide spread throughout the world and impacts both human and animal health. Several distinct ecological clusters and ecotypes of the agent have been established on the basis of various genetic loci. However, information on the genetic variability of A. phagocytophilum isolates in China represents a gap in knowledge. The objective of this study was to determine the prevalence and genetic characterization of A. phagocytophilum in small ruminants in central and southeastern China.
METHODS
The presence of A. phagocytophilum was determined in 421 blood samples collected from small ruminants by PCR. Positive samples were genetically characterized based on 16S rRNA and groEL genes. Statistical analyses were conducted to identify ecotypes of A. phagocytophilum strains, to assess their host range and zoonotic potential.
RESULTS
Out of 421 sampled small ruminants, 106 (25.2%) were positive for A. phagocytophilum. The positive rate was higher in sheep (35.1%, 40/114) than in goats (26.4%, 66/307) (P < 0.05). Sequence analyses revealed that the isolates identified in this study were placed on two separate clades, indicating that two 16S rRNA variants of A. phagocytophilum were circulating in small ruminants in China. However, analysis using obtained groEL sequences in this study formed one cluster, which was separate from other known ecotypes reported in Europe. In addition, a novel Anaplasma sp. was identified and closely related to an isolate previously reported in Hyalomma asiaticum, which clustered independently from all recognized Anaplasma species.
CONCLUSIONS
A molecular survey of A. phagocytophilum was conducted in sheep and goats from ten provinces in central and southeastern China. Two 16S rRNA variants and a new ecotype of A. phagocytophilum were identified in small ruminants in China. Moreover, a potential novel Anaplasma species was reported in goats. Our findings provide additional information on the complexity of A. phagocytophilum in terms of genetic diversity in China.
背景
嗜吞噬细胞无形体在全球广泛传播，对人类和动物健康均有影响。基于各种基因座已确定了该病原体的几个不同生态簇和生态型。然而，关于中国嗜吞噬细胞无形体分离株的遗传变异性信息尚存在知识空白。本研究的目的是确定中国中部和东南部小反刍动物中嗜吞噬细胞无形体的流行情况和遗传特征。
方法
通过聚合酶链反应（PCR）检测从421份小反刍动物血液样本中嗜吞噬细胞无形体的存在情况。基于16S核糖体RNA（rRNA）和groEL基因对阳性样本进行遗传特征分析。进行统计分析以确定嗜吞噬细胞无形体菌株的生态型，评估其宿主范围和人畜共患病潜力。
结果
在421只采样的小反刍动物中，106只（25.2%）嗜吞噬细胞无形体呈阳性。绵羊的阳性率（35.1%，40/114）高于山羊（26.4%，66/307）（P < 0.05）。序列分析表明，本研究中鉴定的分离株位于两个不同的进化枝上，表明嗜吞噬细胞无形体的两种16S rRNA变体在中国小反刍动物中传播。然而，本研究中使用获得的groEL序列分析形成了一个簇，该簇与欧洲报道的其他已知生态型不同。此外，还鉴定出一种新型无形体属物种，它与先前在亚洲璃眼蜱中报道的一种分离株密切相关，该分离株独立于所有公认的无形体属物种聚类。
结论
对中国中部和东南部十个省份的绵羊和山羊进行了嗜吞噬细胞无形体的分子调查。在中国小反刍动物中鉴定出嗜吞噬细胞无形体的两种16S rRNA变体和一种新的生态型。此外，在山羊中报道了一种潜在的新型无形体属物种。我们的研究结果为中国嗜吞噬细胞无形体在遗传多样性方面的复杂性提供了更多信息。
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