State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, Beijing 100071, People's Republic of China.
Vet Microbiol. 2010 Aug 26;144(3-4):405-8. doi: 10.1016/j.vetmic.2010.02.018. Epub 2010 Feb 13.
To identify the prevalence of Anaplasma phagocytophilum in both wild rodents and domestic animals and to make clear the genetic characteristics of the agents from different animals in China, a total of 105 livestock and 159 small rodents were analyzed by real-time-PCR and sequence analysis. The prevalence rate was 6.7% (7/105) and 14.5% (23/159), respectively. The nucleotide sequences of 16S rRNA (rrs) from the positive livestock and rodents were identical to each other. The phylogenetic analysis based on partial A. phagocytophilum p44ESup1 gene revealed that A. phagocytophilum identified in this study was placed on a separate clade distinct from those in other continents. These findings indicated A. phagocytophilum in rodents might be able to infect livestock and intensified the threats of anaplasmosis to livestock in the area. Further studies on public health significance of the agent are worth investigation in future.
为了确定中国野生啮齿动物和家畜中嗜吞噬细胞无形体的流行情况，并明确来自不同动物的病原体的遗传特征，我们通过实时 PCR 和序列分析对 105 种家畜和 159 种小型啮齿动物进行了分析。结果显示，家畜和啮齿动物的阳性率分别为 6.7%（7/105）和 14.5%（23/159）。来自阳性家畜和啮齿动物的 16S rRNA（rrs）核苷酸序列完全相同。基于部分嗜吞噬细胞无形体 p44ESup1 基因的系统发育分析表明，本研究中鉴定的嗜吞噬细胞无形体与其他各大洲的菌株处于不同的分支。这些发现表明，啮齿动物中的嗜吞噬细胞无形体可能能够感染家畜，并加剧该地区家畜类无形体病的威胁。未来值得进一步研究该病原体对公共卫生的意义。
