Ben Said Mourad, Belkahia Hanène, El Mabrouk Narjesse, Saidani Mariem, Ben Hassen Mariem, Alberti Alberto, Zobba Rosanna, Bouattour Skander, Bouattour Ali, Messadi Lilia
Laboratoire de Microbiologie, Ecole Nationale de Médecine Vétérinaire, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, Université de La Manouba, Sidi Thabet, Tunisia.
Laboratoire de Microbiologie, Ecole Nationale de Médecine Vétérinaire, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, Université de La Manouba, Sidi Thabet, Tunisia.
Ticks Tick Borne Dis. 2017 Mar;8(3):412-422. doi: 10.1016/j.ttbdis.2017.01.005. Epub 2017 Jan 16.
Accurate diagnosis of animal and zoonotic diseases, such as granulocytic anaplasmosis, is crucial to estimate risk during control programs. In this study, 16S rRNA nested PCR and RFLP assay were combined to investigate the presence of Anaplasma phagocytophilum and genetically related strains (namely A. phagocytophilum-like 1 and 2) in 936 Tunisian ruminants. By using this method, A. phagocytophilum was not detected in any of the tested animals, while A. phagocytophilum-like 1 and A. phagocytophilum-like 2 were detected at variable prevalence rates in sheep, goats and cattle at coinfection rates respectively of 3.9, 2.5 and 0.5%. Sequence analysis validated RFLP data, and confirmed the co-occurrence of two potentially novel species closely related to A. phagocytophilum in Tunisian ruminants. Phylogeny indicated the presence of genetic variants shared by different ruminant species for each type of A. phagocytophilum-like strains. Results raise concern on the use and interpretation of indirect and direct tests traditionally employed for detecting pathogenic A. phagocytophilum strains in ruminants and in other vertebrates' species, and provide additional background to improve classification of bacterial species closely related to A. phagocytophilum, and to reconstruct their evolutionary history.
准确诊断动物疾病和人畜共患病，如粒细胞无形体病，对于评估防控计划中的风险至关重要。在本研究中，将16S rRNA巢式PCR和RFLP分析相结合，调查了936只突尼斯反刍动物中嗜吞噬细胞无形体及遗传相关菌株（即嗜吞噬细胞无形体样1和2）的存在情况。通过使用这种方法，在任何受试动物中均未检测到嗜吞噬细胞无形体，而在绵羊、山羊和牛中分别以3.9%、2.5%和0.5%的共感染率检测到了嗜吞噬细胞无形体样1和嗜吞噬细胞无形体样2，其流行率各不相同。序列分析验证了RFLP数据，并证实了突尼斯反刍动物中与嗜吞噬细胞无形体密切相关的两个潜在新物种的共存。系统发育分析表明，每种嗜吞噬细胞无形体样菌株在不同反刍动物物种中都存在遗传变异。研究结果引发了人们对传统上用于检测反刍动物和其他脊椎动物物种中致病性嗜吞噬细胞无形体菌株的间接和直接检测方法的使用和解读的关注，并为改进与嗜吞噬细胞无形体密切相关的细菌物种分类以及重建其进化历史提供了更多背景信息。
