Ahmadi Mohammad Hossein, Mirsalehian Akbar, Sadighi Gilani Mohammad Ali, Bahador Abbas, Talebi Malihe
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran; Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Urology. 2017 Feb;100:97-102. doi: 10.1016/j.urology.2016.11.018. Epub 2016 Nov 18.
To elucidate the association between asymptomatic infections caused by Mycoplasma hominis and male infertility and to evaluate the role of antibiotic therapy in the treatment of this failure.
A total of 165 infertile men having abnormal semen parameters (study group) as well as 165 healthy fertile men (control group) were included in this study. Semen samples were taken from all participants and, after analyzing for semen parameters, real-time polymerase chain reaction, microbial culture, and reactive oxygen species (ROS) as well as total antioxidant capacity (TAC) assays were performed. Infected individuals of the study group were treated with antibiotic. One month after the treatment completion, second semen samples were taken and all the tests mentioned were performed. The data were analyzed using SPSS statistical software, version 22.0.
The frequency of M. hominis was significantly higher in the infertile men compared with the fertile ones (14.5% vs 3.6%, P = .001). The mean cycle threshold (C) value was lower in infected infertile men than in infected fertile men (P < .001). All semen parameters, except volume, pH, and viscosity, were improved (P < .05), most of which reached their normal range; leukocytes in seminal fluid were eliminated (P = .04); the level of TAC elevated (P < .001); and the ROS level as well as the ROS-to-TAC ratio reduced after antibiotic treatment (P = .02). Moreover, wives of 14 infected infertile men (58.3%) became pregnant 4 months after the treatment completion.
Our data suggest that asymptomatic infection caused by M. hominis is correlated with male infertility and antibiotic therapy can improve the semen quality and fairly treat the male infertility.
阐明人型支原体引起的无症状感染与男性不育之间的关联，并评估抗生素治疗在治疗这种不育症中的作用。
本研究纳入了165名精液参数异常的不育男性（研究组）以及165名健康的可育男性（对照组）。采集所有参与者的精液样本，在分析精液参数后，进行实时聚合酶链反应、微生物培养、活性氧（ROS）以及总抗氧化能力（TAC）检测。研究组中受感染的个体接受抗生素治疗。治疗结束1个月后，采集第二次精液样本并进行上述所有检测。使用SPSS 22.0统计软件对数据进行分析。
与可育男性相比，不育男性中人型支原体的感染频率显著更高（14.5% 对3.6%，P = 0.001）。感染的不育男性的平均循环阈值（C）值低于感染的可育男性（P < 0.001）。除体积、pH值和粘度外，所有精液参数均有所改善（P < 0.05），其中大多数达到正常范围；精液中的白细胞被清除（P = 0.04）；TAC水平升高（P < 0.001）；抗生素治疗后ROS水平以及ROS与TAC的比值降低（P = 0.02）。此外，14名受感染的不育男性的妻子（58.3%）在治疗结束4个月后怀孕。
我们的数据表明，人型支原体引起的无症状感染与男性不育相关，抗生素治疗可改善精液质量并有效治疗男性不育症。
