在尼日利亚伊巴丹健康孕妇的共生大肠杆菌中鉴定出一种携带bla的新型质粒。
A novel plasmid carrying bla identified in commensal Escherichia coli from healthy pregnant women in Ibadan, Nigeria.
作者信息
Fortini Daniela, Fashae Kayode, Villa Laura, Feudi Claudia, García-Fernández Aurora, Carattoli Alessandra
机构信息
Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
Department of Microbiology, University of Ibadan, Ibadan, Nigeria.
出版信息
J Glob Antimicrob Resist. 2015 Mar;3(1):9-12. doi: 10.1016/j.jgar.2014.12.002. Epub 2014 Dec 31.
The aim of this study was to investigate the molecular characteristics of commensal Escherichia coli producing extended-spectrum β-lactamases and showing fluoroquinolone resistance circulating in a healthy population in Ibadan, Nigeria. In total, 101 faecal samples from healthy pregnant women on the day of admission to hospital were collected and plated on eosin-methylene blue agar supplemented with cefotaxime. Genotyping demonstrated the presence of the bla gene in all of the cefotaxime-resistant isolates (n=32), and there was circulation of prevalent clones. The aac(6')-Ib-cr, qnrS1, qepA1 and qnrB1 genes were identified in several strains. A novel plasmid supporting the spread of the bla, bla and qnrS1 genes was identified in these isolates by complete DNA sequencing.
本研究的目的是调查在尼日利亚伊巴丹健康人群中循环的产超广谱β-内酰胺酶并对氟喹诺酮耐药的共生大肠杆菌的分子特征。总共收集了101份健康孕妇入院当天的粪便样本，并接种在补充有头孢噻肟的伊红美蓝琼脂平板上。基因分型显示所有对头孢噻肟耐药的分离株（n = 32）中均存在bla基因，且有流行克隆的循环。在多个菌株中鉴定出了aac(6')-Ib-cr、qnrS1、qepA1和qnrB1基因。通过全DNA测序在这些分离株中鉴定出了一种支持bla、bla和qnrS1基因传播的新型质粒。
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