尼日利亚拉各斯发热和腹泻患者分离的沙门氏菌中同时存在 bla 和 bla 基因的超广谱β-内酰胺酶：一项横断面研究。
Co-existence of extended-spectrum β-lactamases bla and bla genes in Salmonella species isolated from febrile and diarrhoeagenic patients in Lagos, Nigeria: a cross-sectional study.
机构信息
Department of Microbiology, Faculty of Science, Lagos State University, Lasu Post Office, Ojo, P.O. Box 0001, Lagos, Nigeria.
Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Eur J Med Res. 2023 Jan 2;28(1):3. doi: 10.1186/s40001-022-00960-0.
BACKGROUND
Resistance to different antimicrobial classes by Salmonella species has generated a global public health concern. The spread of extended-spectrum β-lactamases (ESBLs) bla gene variants is also increasing. This study aimed to investigate the antibiotic resistance and the carriage of bla and bla as well as the quinolone resistance gene (qnrB19) among Salmonella species from hospitalised patients in Lagos, Nigeria.
METHODS
In this cross-sectional study from April 2021 to August 2021, a total of 508 samples were collected from hospitalised patients. The samples were subjected to standard microbiological investigation. All the isolates were identified using API 20E kits and real-time polymerase chain reaction (RT-PCR). The in vitro antibiotic susceptibility testing (AST) was investigated using the disk diffusion method. Detection of antibiotic resistance and virulence gene makers was conducted using RT-PCR.
RESULTS
In total, 24 Salmonella species were identified. All the isolates were non-typhoidal Salmonella isolates. None of the isolates screened was S. Typhi and S. Paratyphi. Most of the isolates were susceptible to imipenem, ciprofloxacin, ofloxacin and gentamycin, while a high level of resistance to all cephalosporins, penicillin, and some carbapenems was observed. In total, 79.2% (19/24) of the Salmonella isolates harboured the bla variant including 54.2% (13/24) bla and 12.5% (3/24) bla while co-habitation of bla and bla was observed in 12.5% (3/24) of the isolates, respectively. None of the isolates harboured quinolone-resistant qnrB19 gene and virulence gene stn. However, invA gene was present in 66.7% (16/24) of all isolates.
CONCLUSIONS
This study is considered the first report of bla and bla variants in Salmonella species in Nigeria. The continued existence of cefotaximase (CTX-M)-producing Salmonella within our environment calls for the prudent use of cephalosporins.
背景
沙门氏菌对不同类别的抗菌药物的耐药性引起了全球公共卫生关注。同时，扩展型β-内酰胺酶（ESBLs）bla 基因变体的传播也在增加。本研究旨在调查尼日利亚拉各斯住院患者中沙门氏菌的抗生素耐药性以及 bla 和 bla 以及喹诺酮耐药基因（qnrB19）的携带情况。
方法
在 2021 年 4 月至 2021 年 8 月进行的这项横断面研究中，共从住院患者中采集了 508 份样本。对样本进行了标准微生物学调查。所有分离株均使用 API 20E 试剂盒和实时聚合酶链反应（RT-PCR）进行鉴定。采用纸片扩散法进行体外抗生素药敏试验（AST）。使用 RT-PCR 检测抗生素耐药性和毒力基因标记物。
结果
共鉴定出 24 种沙门氏菌。所有分离株均为非伤寒沙门氏菌。未筛选出 S. Typhi 和 S. Paratyphi。大多数分离株对亚胺培南、环丙沙星、氧氟沙星和庆大霉素敏感，而对所有头孢菌素、青霉素和一些碳青霉烯类药物高度耐药。共有 79.2%（19/24）的沙门氏菌分离株携带 bla 变体，其中 54.2%（13/24）为 bla 和 12.5%（3/24）为 bla，同时在 12.5%（3/24）的分离株中观察到 bla 和 bla 的共存。没有分离株携带耐喹诺酮的 qnrB19 基因和毒力基因 stn。然而，所有分离株中均存在 66.7%（16/24）的 invA 基因。
结论
本研究被认为是尼日利亚首次报道沙门氏菌 bla 和 bla 变体。我们环境中持续存在产头孢噻肟酶（CTX-M）的沙门氏菌，这要求谨慎使用头孢菌素。
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