Morales M A, Holmberg K, Xu Z Q, Cozzari C, Hartman B K, Emson P, Goldstein M, Elfvin L G, Hökfelt T
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11819-23. doi: 10.1073/pnas.92.25.11819.
Indirect immunofluorescence methods using a mouse monoclonal antibody raised to rat choline acetyltransferase (ChAT) revealed dense networks of ChAT-immunoreactive fibers in the superior cervical ganglion, the stellate ganglion, and the celiac superior mesenteric ganglion of the rat. Numerous and single ChAT-immunoreactive cell bodies were observed in the stellate and superior cervical ganglia, respectively. The majority of ChAT-immunoreactive fibers in the stellate and superior cervical ganglia were nitric oxide synthase (NOS) positive. Some ChAT-immunoreactive fibers contained enkephalin-like immunoreactivity. Virtually all ChAT-positive cell bodies in the stellate ganglion were vasoactive intestinal polypeptide (VIP)-positive, and some were calcitonin gene-related peptide (CGRP)-positive. After transection of the cervical sympathetic trunk almost all ChAT- and NOS-positive fibers and most enkephalin- and CGRP-positive fibers disappeared in the superior cervical ganglion. The results suggest that most preganglionic fibers are cholinergic and that the majority of these in addition can release nitric oxide, some enkephalin, and a few CGRP. Acetylcholine, VIP, and CGRP are coexisting messenger molecules in some postganglionic sympathetic neurons.
使用针对大鼠胆碱乙酰转移酶（ChAT）产生的小鼠单克隆抗体的间接免疫荧光方法显示，在大鼠的颈上神经节、星状神经节和腹腔上肠系膜神经节中存在密集的ChAT免疫反应性纤维网络。分别在星状神经节和颈上神经节中观察到大量单个的ChAT免疫反应性细胞体。星状神经节和颈上神经节中的大多数ChAT免疫反应性纤维一氧化氮合酶（NOS）呈阳性。一些ChAT免疫反应性纤维含有脑啡肽样免疫反应性。星状神经节中几乎所有ChAT阳性细胞体血管活性肠肽（VIP）呈阳性，一些降钙素基因相关肽（CGRP）呈阳性。切断颈交感干后，颈上神经节中几乎所有ChAT和NOS阳性纤维以及大多数脑啡肽和CGRP阳性纤维消失。结果表明，大多数节前纤维是胆碱能的，并且其中大多数还可以释放一氧化氮、一些脑啡肽和少量CGRP。乙酰胆碱、VIP和CGRP在一些节后交感神经元中共存作为信使分子。
