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传统检测方法与基于分子的检测方法在吉尔小牛罕见巴贝斯虫病病例中的应用及其治疗管理

Traditional versus molecular based detection of a rare occurrence of babesiosis in Gir calf and its therapeutic management.

作者信息

Maharana Biswa Ranjan, Kumar Binod, Hirani Nitinkumar Devrajbhai

机构信息

Department of Veterinary Parasitology, College of Veterinary Science and A.H., Junagadh Agricultural University (JAU), Junagadh, 362001 Gujarat India.

出版信息

J Parasit Dis. 2016 Dec;40(4):1601-1604. doi: 10.1007/s12639-015-0738-1. Epub 2016 Jan 13.

DOI:10.1007/s12639-015-0738-1
PMID:27876992
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5118364/
Abstract

A 30-day old Gir calf was brought to Veterinary Polyclinic with symptoms of high fever, dullness, dyspnea, pale mucus membrane and haemoglobinuria. Blood sample was collected and microscopic examination of thin blood smear confirmed the case of acute babesiosis. It was further confirmed by polymerase chain reaction that amplified an approximately 410 bp portion of the ssu-rDNA of spp. The calf was managed with diminazene aceturate @5 mg/kg (Berenil) intramuscularly followed by supportive therapy including intravenous infusions. The present study reports a rare case of bovine babesiosis, its clinical variants, diagnosis, hematology and therapeutic management.

摘要

一头30日龄的吉尔小牛被带到兽医综合诊所,出现高烧、精神沉郁、呼吸困难、黏膜苍白和血红蛋白尿等症状。采集血样,薄血涂片显微镜检查确诊为急性巴贝斯虫病病例。通过聚合酶链反应进一步证实,该反应扩增出了 spp. 的小亚基核糖体DNA(ssu-rDNA)约410 bp的片段。对这头小牛肌肉注射了5 mg/kg的乙酰氨基阿维菌素(贝尼尔),随后进行了包括静脉输液在内的支持性治疗。本研究报告了一例罕见的牛巴贝斯虫病病例、其临床变体、诊断、血液学及治疗处理情况。

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本文引用的文献

1
Babesia bigemina infection in a 14-day old Jersey crossbred calf: a case report.一头14日龄泽西杂交犊牛的双芽巴贝斯虫感染:病例报告
J Parasit Dis. 2015 Jun;39(2):264-5. doi: 10.1007/s12639-013-0338-x. Epub 2013 Jun 23.
2
Molecular prevalence of Babesia bigemina and Trypanosoma evansi in dairy animals from Punjab, India, by duplex PCR: a step forward to the detection and management of concurrent latent infections.印度旁遮普邦双 PCR 检测牛 Babesia bigemina 和 T. evansi 分子流行率:检测和管理同时潜伏感染的重要一步。
Biomed Res Int. 2013;2013:893862. doi: 10.1155/2013/893862. Epub 2013 Aug 29.
3
Intra-uterine Infection with Babesia bovis in a 2-day-old Calf.一头2日龄犊牛发生牛巴贝斯虫子宫内感染。
J Vet Med B Infect Dis Vet Public Health. 2003 Mar;50(2):60-2. doi: 10.1046/j.1439-0450.2003.00597.x.
4
The age-related immunity in cattle to Babesia bovis infection involves the rapid induction of interleukin-12, interferon-gamma and inducible nitric oxide synthase mRNA expression in the spleen.牛对牛巴贝斯虫感染的年龄相关免疫涉及脾脏中白细胞介素-12、γ-干扰素和诱导型一氧化氮合酶mRNA表达的快速诱导。
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A subtropical case of human babesiosis.一例亚热带地区的人类巴贝斯虫病病例。
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