Bal Mandeep Singh, Mahajan Vishal, Filia Gursimran, Kaur Paramjit, Singh Amarjit
Animal Disease Research Centre, Guru Angad Dev Veterinary & Animal Sciences University, Ludhiana, Punjab, India.
Department of Veterinary Parasitology, Guru Angad Dev Veterinary & Animal Sciences University, Ludhiana, Punjab, India.
Vet World. 2016 Dec;9(12):1370-1374. doi: 10.14202/vetworld.2016.1370-1374. Epub 2016 Dec 6.
The aim of the present study was to diagnose severe outbreaks of bovine babesiosis in Punjab state, in the year 2015 and to suggest control and preventive measures to animal owners.
Mortality of animals was recorded in two cattle herd comprising a total of 465 cattle in Sangrur (n=125) and Faridkot (n=340) districts. There was a history of purchase of animals at one farm. 23 blood samples were collected from diseased (n=15) and healthy animals (n=8) for hematological analysis, parasitological, and polymerase chain reaction (PCR)-based diagnosis. Ticks were also collected from animals for identification.
Out of 465 cattle at risk, 28 were critically ill and 14 died of disease with morbidity, mortality, and case fatality rate of 6.02%, 3.01%, and 50.00%, respectively. Clinical signs and necropsy findings were suggestive of babesiosis. Ticks collected from both the outbreaks were identified as . Thin blood smears from infected animals (especially with clinical sign of hemoglobinuria) were found positive for organisms; however, molecular diagnosis (PCR) further confirmed the disease. Animals were successfully treated with diminazene aceturate, hematinics, and antipyretics.
Two fatal outbreaks of babesiosis in cattle were diagnosed with application of conventional parasitological, hematological, and molecular diagnostic techniques. PCR was found to be far more sensitive in detecting the disease, especially in latent infections. Animal owners were advised to follow quarantine measures before mixing new animals in the herd and strategic acaricidal treatments for effective tick control.
本研究旨在诊断2015年旁遮普邦严重的牛巴贝斯虫病疫情，并向动物所有者提出控制和预防措施。
记录了桑格鲁尔（n = 125）和法里德科特（n = 340）两个牛群中动物的死亡率，这两个牛群共有465头牛。其中一个农场有购买动物的历史。从患病动物（n = 15）和健康动物（n = 8）采集了23份血样，用于血液学分析、寄生虫学和基于聚合酶链反应（PCR）的诊断。还从动物身上采集蜱虫进行鉴定。
在465头有风险的牛中，28头病情危急，14头因病死亡，发病率、死亡率和病死率分别为6.02%、3.01%和50.00%。临床症状和尸检结果提示为巴贝斯虫病。从两次疫情中采集的蜱虫被鉴定为 。从感染动物（尤其是有血红蛋白尿临床症状的动物）采集的薄血涂片发现 生物体呈阳性；然而，分子诊断（PCR）进一步确诊了该病。动物用乙酰氨基苯磺酰脒、补血药和解热药成功治疗。
应用传统寄生虫学、血液学和分子诊断技术诊断出两起致命的牛巴贝斯虫病疫情。发现PCR在检测该病时灵敏度高得多，尤其是在潜伏感染中。建议动物所有者在将新动物混入牛群之前采取检疫措施，并进行战略性杀螨处理以有效控制蜱虫。
