Haque Md Amranul, Nagaoka Masato, Hexig Bayar, Akaike Toshihiro
Department of Biomolecular Engineering, Gaduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 226-8501 Yokohama, Japan.
Department of Biomolecular Engineering, Gaduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 226-8501 Yokohama, Japan; Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Sci Technol Adv Mater. 2010 Feb 26;11(1):014106. doi: 10.1088/1468-6996/11/1/014106. eCollection 2010 Feb.
Nanobiomaterials can play a central role in regenerative medicine and tissue engineering by facilitating cellular behavior and function, such as those where extracellular matrices (ECMs) direct embryonic stem (ES) cell morphogenesis, proliferation, differentiation and apoptosis. However, controlling ES cell proliferation and differentiation using matrices from natural sources is still challenging due to complex and heterogeneous culture conditions. Moreover, the systemic investigation of the regulation of self-renewal and differentiation to lineage specific cells depends on the use of defined and stress-free culture conditions. Both goals can be achieved by the development of biomaterial design targeting ECM or growth factors for ES cell culture. This targeted application will benefit from expansion of ES cells for transplantation, as well as the production of a specific differentiated cell type either by controlling the differentiation in a very specific pathway or by elimination of undesirable cell types.
纳米生物材料可通过促进细胞行为和功能，在再生医学和组织工程中发挥核心作用，例如细胞外基质（ECM）指导胚胎干细胞（ES）的形态发生、增殖、分化和凋亡。然而，由于复杂且异质的培养条件，利用天然来源的基质控制ES细胞的增殖和分化仍然具有挑战性。此外，对自我更新以及向谱系特异性细胞分化的调控进行系统性研究，依赖于使用明确且无应激的培养条件。这两个目标都可以通过开发针对ES细胞培养的ECM或生长因子的生物材料设计来实现。这种靶向应用将受益于用于移植的ES细胞的扩增，以及通过在非常特定的途径中控制分化或消除不需要的细胞类型来产生特定的分化细胞类型。
