Witecka Agnieszka, Yamamoto Akiko, Dybiec Henryk, Swieszkowski Wojciech
Biomaterials Unit, Biometal Group, National Institute for Materials Science, Tsukuba, Ibaraki, 305-0044, Japan; Faculty of Materials Science and Engineering, Warsaw University of Technology, 141 Woloska Street, Warsaw, 02-507, Poland.
Biomaterials Unit, Biometal Group, National Institute for Materials Science, Tsukuba, Ibaraki, 305-0044, Japan.
Sci Technol Adv Mater. 2012 Dec 13;13(6):064214. doi: 10.1088/1468-6996/13/6/064214. eCollection 2012 Dec.
Mg alloys with high Al contents have superior corrosion resistance in aqueous environments, but poor cytocompatibility compared to that of pure Mg. We have silanized the cast AZ91 alloy to improve its cytocompatibility using five different silanes: ethyltriethoxysilane (S1), 3-aminopropyltriethoxysilane (S2), 3-isocyanatopyltriethoxysilane (S3), phenyltriethoxysilane (S4) and octadecyltriethoxysilane (S5). The surface hydrophilicity/hydrophobicity was evaluated by water contact angle measurements. X-ray photoelectron analysis was performed to investigate the changes in surface states and chemical composition. All silane reagents increased adsorption of the albumin to the modified surface. cytocompatibility evaluation revealed that silanization improved cell growth on AZ91 modified by silane S1. Measurement of the concentration of Mg ions released during the cell culture indicated that silanization does not affect substrate degradation.
高铝含量的镁合金在水性环境中具有优异的耐腐蚀性，但与纯镁相比，其细胞相容性较差。我们使用五种不同的硅烷对铸造AZ91合金进行了硅烷化处理，以提高其细胞相容性：乙基三乙氧基硅烷（S1）、3-氨丙基三乙氧基硅烷（S2）、3-异氰酸酯丙基三乙氧基硅烷（S3）、苯基三乙氧基硅烷（S4）和十八烷基三乙氧基硅烷（S5）。通过水接触角测量来评估表面亲水性/疏水性。进行X射线光电子分析以研究表面状态和化学成分的变化。所有硅烷试剂都增加了白蛋白在改性表面的吸附。细胞相容性评估表明，硅烷化处理改善了硅烷S1改性的AZ91表面上的细胞生长。细胞培养过程中释放的镁离子浓度测量表明，硅烷化处理不影响基体降解。
