Cicco Stefania Roberta, Vona Danilo, Gristina Roberto, Sardella Eloisa, Ragni Roberta, Lo Presti Marco, Farinola Gianluca Maria
Italian National Council for Research-Institute for the Chemistry of OrganoMetallic Compounds (CNR-ICCOM)-Bari, Bari 70126, Italy.
Department of Chemistry, Università degli Studi di Bari Aldo Moro, Bari 70121, Italy.
Bioengineering (Basel). 2016 Dec 16;3(4):35. doi: 10.3390/bioengineering3040035.
In the past decade, mesoporous silica nanoparticles (MSNs) with a large surface area and pore volume have attracted considerable attention for their application in drug delivery and biomedicine. Here we propose biosilica from diatoms as an alternative source of mesoporous materials in the field of multifunctional supports for cell growth: the biosilica surfaces were chemically modified by traditional silanization methods resulting in diatom silica microparticles functionalized with 3-mercaptopropyl-trimethoxysilane (MPTMS) and 3-aminopropyl-triethoxysilane (APTES). Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analyses revealed that the -SH or -NH₂ were successfully grafted onto the biosilica surface. The relationship among the type of functional groups and the cell viability was established as well as the interaction of the cells with the nanoporosity of frustules. These results show that diatom microparticles are promising natural biomaterials suitable for cell growth, and that the surfaces, owing to the mercapto groups, exhibit good biocompatibility.
在过去十年中，具有大表面积和孔体积的介孔二氧化硅纳米颗粒（MSNs）因其在药物递送和生物医学中的应用而备受关注。在此，我们提出将硅藻中的生物二氧化硅作为细胞生长多功能载体领域中介孔材料的替代来源：通过传统硅烷化方法对生物二氧化硅表面进行化学修饰，得到用3-巯基丙基三甲氧基硅烷（MPTMS）和3-氨丙基三乙氧基硅烷（APTES）功能化的硅藻二氧化硅微粒。傅里叶变换红外光谱和X射线光电子能谱分析表明，-SH或-NH₂成功接枝到生物二氧化硅表面。建立了官能团类型与细胞活力之间的关系以及细胞与硅藻壳纳米孔隙率的相互作用。这些结果表明，硅藻微粒是适用于细胞生长的有前景的天然生物材料，并且由于巯基的存在，其表面具有良好的生物相容性。
