Tsai Tsung-Ting, Shen Shu-Wei, Cheng Chao-Min, Chen Chien-Fu
Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Taipei 105, Taiwan; Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan 333, Taiwan.
Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
Sci Technol Adv Mater. 2013 Aug 6;14(4):044404. doi: 10.1088/1468-6996/14/4/044404. eCollection 2013 Aug.
A colorimetric sensing strategy employing gold nanoparticles and a paper assay platform has been developed for tuberculosis diagnosis. Unmodified gold nanoparticles and single-stranded detection oligonucleotides are used to achieve rapid diagnosis without complicated and time-consuming thiolated or other surface-modified probe preparation processes. To eliminate the use of sophisticated equipment for data analysis, the color variance for multiple detection results was simultaneously collected and concentrated on cellulose paper with the data readout transmitted for cloud computing via a smartphone. The results show that the 2.6 nM tuberculosis mycobacterium target sequences extracted from patients can easily be detected, and the turnaround time after the human DNA is extracted from clinical samples was approximately 1 h.
一种采用金纳米颗粒和纸质检测平台的比色传感策略已被开发用于结核病诊断。未修饰的金纳米颗粒和单链检测寡核苷酸被用于实现快速诊断，无需复杂且耗时的硫醇化或其他表面修饰探针制备过程。为了避免使用复杂设备进行数据分析，将多个检测结果的颜色变化同时收集并集中在纤维素纸上，通过智能手机将数据读数传输至云计算进行分析。结果表明，从患者身上提取的2.6 nM结核分枝杆菌靶序列能够轻松被检测到，从临床样本中提取人类DNA后的周转时间约为1小时。
