CIGMH/DCV, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, Caparica, Portugal.
Clin Microbiol Infect. 2010 Sep;16(9):1464-9. doi: 10.1111/j.1469-0691.2009.03120.x.
Members of the Mycobacterium tuberculosis complex (MTC) are causative agents of human and animal tuberculosis. This complex encompasses several phylogenetically related species, including M. tuberculosis, the main aetiological agent of human tuberculosis, and Mycobacterium bovis, the causative agent of bovine tuberculosis, a relevant worldwide zoonosis. Clear epidemiological evaluation of appropriate and effective treatment requires unambiguous differentiation between MTC members. Routine diagnosis has been increasingly relying on the molecular identification of MTC members. In the present study, we report the use of a gold nanoparticle-based approach for the sensitive, specific and fast identification of MTC and for the differentiation of M. bovis and M. tuberculosis using the gyrB locus as target. This gold nanoprobe strategy relies on the colorimetric differentiation of specific DNA sequences based on differential aggregation profiles in the presence or absence of specific target hybridization. Three nanoprobes were designed and successfully used for the specific identification of members of MTC, M. bovis and M. tuberculosis.
分枝杆菌复合群（MTC）成员是人类和动物结核病的病原体。该复合群包括几个系统发育上相关的物种，包括结核分枝杆菌，这是人类结核病的主要病原体，以及牛分枝杆菌，这是牛结核病的病原体，是一种相关的世界性人畜共患病。明确的流行病学评估适当和有效的治疗需要明确区分 MTC 成员。常规诊断越来越依赖于 MTC 成员的分子鉴定。在本研究中，我们报告了一种基于金纳米粒子的方法，用于敏感、特异性和快速鉴定 MTC，并使用gyrB 基因座作为靶标区分牛分枝杆菌和结核分枝杆菌。这种金纳米探针策略依赖于基于特定 DNA 序列的比色区分，这些序列在存在或不存在特定目标杂交的情况下具有不同的聚集谱。设计并成功使用了三种纳米探针来特异性鉴定 MTC、牛分枝杆菌和结核分枝杆菌成员。
