Wadley Jessica J, Fordham Damien A, Thomson Vicki A, Ritchie Euan G, Austin Jeremy J
Australian Centre for Ancient DNAUniversity of AdelaideNorth TerraceAdelaideSouth Australia5005Australia; Environment Institute and School of Biological SciencesUniversity of AdelaideNorth TerraceAdelaideSouth Australia5005Australia.
Environment Institute and School of Biological Sciences University of Adelaide North Terrace Adelaide South Australia 5005 Australia.
Ecol Evol. 2016 Oct 14;6(22):8050-8061. doi: 10.1002/ece3.2381. eCollection 2016 Nov.
The distribution of antilopine wallaroo, , is marked by a break in the species' range between Queensland and the Northern Territory, coinciding with the Carpentarian barrier. Previous work on revealed limited genetic differentiation between the Northern Territory and Queensland populations across this barrier. The study also identified a number of divergent lineages in the Northern Territory, but was unable to elucidate any geographic structure. Here, we re-examine these results to (1) determine phylogeographic patterns across the range of and (2) infer the biogeographic barriers associated with these patterns. The tropical savannahs of northern Australia: from the Cape York Peninsula in the east, to the Kimberley in the west. We examined phylogeographic patterns in using a larger number of samples and three mtDNA genes: NADH dehydrogenase subunit 2, cytochrome b, and the control region. Two datasets were generated and analyzed: (1) a subset of samples with all three mtDNA regions concatenated together and (2) all samples for just control region sequences that included samples from the previous study. Analysis included generating phylogenetic trees based on Bayesian analysis and intraspecific median-joining networks. The contemporary spatial structure of mtDNA lineages revealed five shallow clades and a sixth, divergent lineage. The genetic differences that we found between Queensland and Northern Territory samples confirmed the split in the geographic distribution of the species. We also found weak genetic differentiation between Northern Territory samples and those from the Kimberley region of Western Australia, possibly due to the Kimberley Plateau-Arnhem Land barrier. Within the Northern Territory, two clades appear to be parapatric in the west, while another two clades are broadly sympatric across the Northern Territory. MtDNA diversity of revealed an unexpectedly complex evolutionary history involving multiple sympatric and parapatric mtDNA clades across northern Australia. These phylogeographic patterns highlight the importance of investigating genetic variation across distributions of species and integrating this information into biodiversity conservation.
草原袋鼠的分布以昆士兰州和北领地之间该物种分布范围的间断为特征，这与卡奔塔利亚屏障相吻合。此前对草原袋鼠的研究表明，跨越这一屏障的北领地和昆士兰州种群之间的遗传分化有限。该研究还在北领地发现了一些不同的谱系，但未能阐明任何地理结构。在此，我们重新审视这些结果，以（1）确定草原袋鼠分布范围内的系统地理学模式，以及（2）推断与这些模式相关的生物地理学屏障。澳大利亚北部的热带稀树草原：从东部的约克角半岛到西部的金伯利地区。我们使用大量样本和三个线粒体DNA基因（烟酰胺腺嘌呤二核苷酸脱氢酶亚基2、细胞色素b和控制区）来研究草原袋鼠的系统地理学模式。生成并分析了两个数据集：（1）将所有三个线粒体DNA区域串联在一起的样本子集，以及（2）仅针对控制区序列的所有样本，其中包括来自先前研究的样本。分析包括基于贝叶斯分析生成系统发育树和种内中位数连接网络。草原袋鼠线粒体DNA谱系的当代空间结构揭示了五个浅分支和第六个不同的谱系。我们在昆士兰州和北领地的草原袋鼠样本之间发现的遗传差异证实了该物种地理分布的分裂。我们还发现北领地样本与西澳大利亚金伯利地区样本之间的遗传分化较弱，这可能是由于金伯利高原 - 阿纳姆地屏障所致。在北领地内，西部有两个分支似乎是邻域分布，而另外两个分支在整个北领地广泛同域分布。草原袋鼠的线粒体DNA多样性揭示了一个意想不到的复杂进化历史，涉及澳大利亚北部多个同域和邻域的线粒体DNA分支。这些系统地理学模式凸显了调查物种分布范围内遗传变异并将此信息纳入生物多样性保护的重要性。
