Ke L, Xu S-B, Wang J, Jiang X-L, Xu M-Q
Department of Thoracic Surgery, Anhui Provincial Hospital, No. 17 Lujiang Road, Hefei, 230000, China.
Clin Transl Oncol. 2017 May;19(5):599-605. doi: 10.1007/s12094-016-1572-3. Epub 2016 Nov 22.
Long non-coding RNAs (lncRNAs) have been demonstrated to act as a critical regulator in the processes of tumor biology. In this study, whether lncRNA-ATB is a potential indicator for non-small cell lung cancer (NSCLC) was investigated and its biological function in NSCLC was also determined.
The expression levels of lncRNA-ATB in NSCLC tissues and cell lines were measured. A549 cell line was explored to investigate the functions of lncRNA-ATB in NSCLC.
Real-time PCR results showed that lncRNA-ATB expression was up-regulated in both in NSCLC tissues and cell lines. High lncRNA-ATB expression in tumor tissue was associated with larger tumor size, lymph node metastasis, and distant metastasis in patients with NSCLC, respectively. In addition, the patients with high expression of lncRNA-ATB presented a lower survival probability. In vitro experiments showed that down-regulation of lncRNA-ATB promoted the cell apoptosis, whereas inhibited the cell viability, cell migration, and cell invasion.
High expression of lncRNA-ATB indicated a poor prognosis and led to the cell proliferation and metastasis in NSCLC.
长链非编码RNA（lncRNAs）已被证明在肿瘤生物学过程中起关键调节作用。在本研究中，探讨了lncRNA-ATB是否为非小细胞肺癌（NSCLC）的潜在指标，并确定了其在NSCLC中的生物学功能。
检测NSCLC组织和细胞系中lncRNA-ATB的表达水平。利用A549细胞系研究lncRNA-ATB在NSCLC中的功能。
实时PCR结果显示，lncRNA-ATB在NSCLC组织和细胞系中均上调。肿瘤组织中lncRNA-ATB高表达分别与NSCLC患者的肿瘤体积较大、淋巴结转移和远处转移相关。此外，lncRNA-ATB高表达的患者生存概率较低。体外实验表明，lncRNA-ATB的下调促进细胞凋亡，而抑制细胞活力、细胞迁移和细胞侵袭。
lncRNA-ATB高表达表明NSCLC预后不良，并导致细胞增殖和转移。
